期 刊 ：中国中药杂志 2008年 33卷 19期 页码：2207.
Keywords:Chrysanthemum morifolium, cultivars, essential oil, GC-MS,
摘 要 ：目的：分析6种栽培类型安徽产菊花(早贡菊、晚贡菊、黄药菊、滁菊、小亳菊和大亳菊)挥发油成分，为其质量评价提供科学依据。方法：采用水蒸气蒸馏法提取6种栽培类型菊花挥发油，用GC毛细管色谱柱进行分析，归一化法测定其相对含量，GC-MS鉴定化学成分。结果：从早贡菊、晚贡菊、黄药菊、滁菊、小亳菊和大亳菊挥发油中分别检出247，226，246，182，216和122个色谱峰，分别鉴定了75，54，78，50，53和43个化合物，分别占挥发油总量的85.67%，82.80%，81.38%，73.22%，71.51%和72.87%。结论：除滁菊外，其他5种菊花中单萜含量较倍半萜高；早贡菊和晚贡菊在挥发油成分上没有差异，醋酸马鞭草醇酯为两者的主要成分，分别占挥发油总量的32.10%和37.85%；1R-樟脑是黄药菊的主要成分，其次是甜没药醇氧化物A，分别占挥发油总量的28.70%和12.58%；β-芹子烯和龙脑是滁菊的主要成分，分别占挥发油总量的17.85%和12.84%；桉叶素是小亳菊的绝对主要成分，占挥发油总量的21.33%；大亳菊中马鞭草烯氧化产物占挥发油总量的25.32%，菊油环酮占挥发油总量的8.26%。从整体来看，安徽产6种栽培类型菊花挥发油中共有成分为樟脑烯、龙脑、龙脑醋酯、1R-樟脑、左旋-4-萜品醇、α-松油醇、桉叶素、顺式-石竹烯、氧化石竹烯、杜松脑、β-倍半水芹烯、α-姜黄烯和β-金合欢烯等。这些结果为安徽产菊花栽培类型的确立提供了化学方面的佐证，亦为其质量评价提供了物质基础。
Abstract:Objective: To analyze the chemical components of the essential oil extracted from six cultivars‘ Juhua(Zaogongju, Wangongju, Huangyaoju, Chuju, Xiaoboju and Daboju) produced in Anhui province of China, which were breed from Chrysanthemum morifolium, and to provide scientific basis for quality control. Method: The essential oils of the six cultivars‘ Juhua were extracted by water-steam distillation and separated by GC capillary column chromatography. The components were quantitatively determined with normalization method, and identified by GC-MS. Result: From Zaogongju, Wangongju, Huangyaoju, Chuju, Xiaoboju and Daboju, 247, 226, 246, 182, 216 and 122 chromatographic peaks were detected, among them 75, 54, 78, 50, 53 and 43 components were identified,which were composed of 85.67%, 82.80%, 81.38%, 73.22%, 71.51% and 72.87% of the total essential oil, respectively. Conclusion: Monoterpenoid compounds were higher more than sesquiterpenoid compounds in the five juhua except for Chuju. There was no difference in the constituents of essential oil of Zaogongju and Wangongju. And verbenyl acetate was the main chemical constituent and composed of 32.10% and 37.85% of the total essential oil, respectively. (1R)-Camphor and bisabolol oxide A were the main chemical constituents in Huangyaoju, which were composed of 28.70% and 12.58% of the total essential oil, respectively. β-Selinene and borneol were the main chemical constituents in Chuju, which were composed of 17.85% and 12.84% of the total essential oil,respectively. Eucalyptol was the main chemical constituent in Xiaoboju,which were composed of 21.33%. Verbene oxides and chrysanthenone were composed of 25.32% and 8.26% of the total essential oil, respectively, in the Daboju. The common chemical constituents were camphene,borneol, bornyl aceate, (1R)-camphor, (-)-4-terpineol, α-terpineol,eucalyptol, cis-caryophyllene, caryophyllene oxide, juniper camphor, β-sesquiphellandrene, α-curcumene and β-farnesene in the six cultivars‘ Juhua produced in Anhui province of China. The resultant data provided the further evidences to distinguish Juhua‘ cultivars and scientific basis for quality control of Juhua.