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期 刊 ：中国中药杂志 2008年 33卷 08期 页码：939.

关键词：青藤碱；过氧化氢；心肌细胞；凋亡；

Keywords:sinomenine, hydrogen peroxide, cardiomyocyte, apoptosis,

摘 要 ：目的:探讨青藤碱对H₂O₂诱导乳鼠心肌细胞凋亡的影响及其可能的作用机制。方法:在原代培养的SD大鼠乳鼠心肌细胞上建立H₂O₂损伤模型,观察不同剂量青藤碱(10,30,100μmol.L^-1)对心肌细胞凋亡率、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、乳酸脱氢酶(LDH)活性及NF-κB蛋白表达的影响。结果:H₂O₂组与空白对照组相比,心肌细胞凋亡率显著增加(P<0.01),并随着时间的延长其凋亡率不断增高;青藤碱明显抑制H₂O₂所诱导各个时间段的心肌细胞凋亡率(P<0.01),降低MDA含量,增加SOD,LDH活性,抑制NF-κB蛋白表达。结论:青藤碱对H₂O₂诱导的乳鼠心肌细胞凋亡有抑制作用,其作用机制可能与其抗脂质氧化、抑制心肌细胞表达NF-κB有关。

Abstract:Objective:To study the effects of sinomenine on apoptosis in cutured neonatal rat cardiomyocytes induced by H₂O₂ and its possible mechanism.Method:H₂O₂ was used to build an oxidative stress-induced injury model in neonatal rat cardiomyocytes after being treated with sinomenine(10,30,100 μmol·L^-1),the apoptosis rate,the content of malondialdehyde(MDA),the activity of superoxide dimutase(SOD),the activity of lactate dehydrogenase(LDH) and expression of NF-κB protein of the Cardiomyocytes were examined.Result:Compared with the model group,the apoptosis rate and the content of MDA,LDH decreased greatly(P<0.01),and the activity of SOD increased distinctly(P<0.01) after being treated by sinomenine(10,30,100 μmol·L^-1).Conclusion:Sinomenine can inhibit the apoptosis induced by H₂O₂ in neonatal rat cardiomyocytes.The protective mechanism could be related to its ability to reduce lipid pexosidation and to inhibit cardiomyocyte expression of NF-κB protein.

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