Abstract:Objective: To study the chemical constituents in the aerial part of Acalypha australis. Method: The aerial part of Acalypha australis were extracted with EtOH at room temperature. The compounds were separated by various kinds of chromatograpic methods and their structures were identified on the basis of spectroscopic techniques. Result: Eleven compounds have been isolated and identified: emodin (1), β-sitosterol (2), loliolide (3), 2, 6-dimethoxy-1, 4-benzoquinone (4), nicotinic acid (5), protocatechuic acid (6), daucosterol (7), gallic acid (8), rutin (9), succinic acid (10), brevifolin (11). Conclusion: Compounds 1, 3-5, 10 and 11 were obtained from A. australis for the first time.