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期 刊 ：中国中药杂志 2007年 32卷 10期 页码：912.

关键词：大孔吸附树脂；莲心；生物碱；

Keywords:macroporous adsorption resin, Lotus plumule, alkaloids,

摘 要 ：目的：用大孔吸附树脂对莲心生物碱进行分离纯化研究。方法：以吸附量、洗脱率及甲基莲心碱的含量为考察指标,选择纯化工艺。结果：LSA-5B型大孔吸附树脂对莲心生物碱具有较大吸附量。最佳吸附条件是：上样液质量浓度在0.125 g·mL^-1(相当于原生药),洗脱方法为先以纯化水除去杂质,再以50%乙醇洗脱,收集50%乙醇洗脱部分。结论：该法简单可行,适合工业生产。

Abstract:Objective: To explore the optimal separation of the alkaloids from Lotus plumule by selecting appropriate macroporous adsorption resins. Method: To evaluated the separating efficiency by measuring the adsorption ratio, eluting ratio and the concentration of neferine. Result: The LSA-5B macroporous adsorption resin prolided the optimal separating efficiency. The best adsorption capacity was achieved with the following conditions：the concentration of extact liquid was 0.125 g·mL^-1 (equivalence to raw material),then the resin was washed by water to remove impurity and alkaloids were desorbed by 50% ethanol. Conclusion: This method is simple, feasible and suitable for industry production.

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