Abstract:Objective: To purify salvianolic acids by macroreticular resin,then mensurate the contents of salvianolic acids and analyse the chromatogram with HPLC. Method: Make salvianolic acids with macroreticular resin;mensurate the content of Salvianolic acids with UV spestrophotometry: the control compound is protocaechuic aldehyde, and the wavelength is 281 nm. Analysis the chromatogram with HPLC, and compare the chromatogram in different technics: zorbax ODS column(4.6 mm×250 mm, 5 μm), mobilephase: 1% aceticacid-water and methanol in different proportions, the wavelength is 281 nm. Result: The contents of salvianolic acids is 53.8%;HPLC chromatogram indicate that the method is reasonable to make salvianolic acids. Conclusion: Determination of contents and HPLC chromatogram can control the quality of Salvianolic acids more accurately.