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期 刊 ：中国中药杂志 2005年 30卷 12期 页码：885.

关键词：丹参；丹参酮ⅡA；原儿茶醛；金属离子；

Keywords:Salvia niltiorrhiza, tanshinone ⅡA, protocatechuic aldehyde, metal cations,

摘 要 ：目的:研究金属离子对丹参不定根生长和丹参酮ⅡA、原儿茶醛含量的影响。方法:利用组织培养技术培养丹参不定根,并用HPLC测定Fe²⁺,Cu²⁺,Zn²⁺,Mn²⁺,Mg²⁺不同含量处理时丹参酮Ⅱ_A和原儿茶醛的含量。结果:基本MS培养基的Fe²⁺和Mn²⁺含量即可满足不定根生长,较高含量的Cu²⁺和Mg²⁺促进不定根增殖,zn²⁺对丹参不定根生长的影响并不十分显著。Cu²⁺和Zn²⁺抑制原儿茶醛合成,适量的Fe²⁺和Mg²⁺可以促进原儿茶醛的合成,而较高含量的Mn²⁺更利于原儿茶醛的合成。低含量的Cu²⁺,Zn²⁺,Mg²⁺以及较高含量的Fe²⁺和Mn²⁺促进丹参酮ⅡA的合成。结论:金属离子对丹参不定根生长和次生代谢物合成有显著影响。

Abstract:Objective: To study the effects of mineral cations on the growth of Salvia niltiorrhiza adventitious roots and the accumulation of tanshinone Ⅱ_A and protocatechuic aldehyde. Method: The adventitious roots were cultured under different concentrations of metal cations and the contents of tanshinone Ⅱ_A and protocatechuic aldehyde were determined by HPLC. Result: Fe²⁺ and Mn²⁺ in MS medium are suitable for adventitious root growth, but Cu²⁺ and Mg²⁺ in high concentration could improve root proliferation, and Zn²⁺ has no obvious effect on root growth. As for tanshinone Ⅱ_A and protocatechuic aldehyde biosynthesis, Cu²⁺ and Zn²⁺ would inhibit the biosynthesis of protocatechuic aldehyde, furthermore, proper Fe²⁺ and Mg²⁺ concentration could advance the biosynthesis of protocatechuic aldehyde, whereas, Mn²⁺ in high concentration could accelerate the biosynthesis of protocatechuic aldehyde. Cu²⁺ ,Zn²⁺ , Mg²⁺ in low concentration and Fe²⁺ and Mn²⁺ in high concentration would all advance the biosynthesis of tanshinone Ⅱ_A. Conculsion: The mineral cations have obvious effects on the secondary metabolites biosynthesis in adventitious root culture of S. niltiorrhiza.

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