---

期 刊 ：中国中药杂志 2006年 31卷 24期 页码：2067.

关键词：慢性应激；行为学；雌二醇；千金妇宝；

Keywords:chronic stress, behavior, estradiol, QJFB,

摘 要 ：目的：探讨千金妇宝对慢性应激雌性大鼠行为学和血清雌二醇水平的影响。方法：雌性Wistar大鼠(2月龄)24只,随机分为正常组、模型组和千金妇宝组(0.7 g·kg^-1·d^-1),每组8只,药物组及模型组分别接受21 d慢性轻度不可预见性应激,药物组以千金妇宝进行灌胃,正常组和模型组均以生理盐水灌胃。3组均于应激前、应激结束后进行旷场试验,观察雌性大鼠行为学改变,并用放射免疫法检测血清雌二醇的含量。结果：与正常组相比,模型组雌性大鼠旷场实验中,水平运动次数和垂直运动次数明显减少,血清雌二醇水平明显降低(P<0.05),而千金妇宝药物组大鼠的水平运动次数、垂直运动次数和血清雌二醇水平与正常组比无明显差异。结论：慢性应激可引起雌性大鼠雌二醇水平降低,活动行为和探究行为减少；千金妇宝可维持雌激素的水平,并能改善慢性应激大鼠的行为学表现,可以推测千金妇宝具有应激保护作用。

Abstract:Objective： To investigate the effect of Qianjin Fubao (QJFB) on behavior and estradiol level in femal chronic stress model rats. Method: Twenty four female Wistar rats (2 month old) were evenly randomized into normal control, animal model and QJFB (0.7 g·kg^-1·d^-1) group. The QIFB group and the stress group were exposed to a chronic unpredictable stress for 21 days. Rats of the QJFB group received perfusion of Qianjin Fubao, and rats of stress and control group were perfused with normal saline. The behavior of three groups were determined with the method of Open-field before and after right stress respectively. Serum level of estradiol was detected with radioim munoassay. Result: The behavioral score and the serum level of estradiol of the stressed group were significantly lower than those of the control group after stress (P<0.05). There were no significant differences of behavioral score and the serum level of estradiol between QJFB group and control group. Conclusion: The chronic unpredictable stress can induce the stressful change of behavior, and QJFB may recover the rats‘ abnormal behavior and improve the serum level of estradiol. QJFB may have protective effect on stress.

---