Abstract:Objective: To select the best preparation method of vincristine transfersomes( VCR-T) and predict its possibility of being a new formulation of VCR. Method: Orthogonal design was used to optimize the preparation methods on the basis of single factor pretests; and the permeation tests in vitro were performed in modified Franz diffusion cells.Result: The optimum formula was: pH was equal to 7.3, the ratio of lecithin to sodium deoxycholate is 70/20, the weight of VCR is 10 mg, hydrating time is 30 minutes. The optimized solution was light yellow and transparent colloid solution. The VCR-T are spherical and smooth with average diameters of 94 run and an encapsulation ratio of 90% . The test in vitro showed that VCR-T could permeat through mouse skin at zero rate with the cumulative penetrating quality amounting to 63.8%. Conclusion: Transfersomes may become a promising carrier of VCR for clinic use.