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期 刊 ：中国中药杂志 2005年 30卷 16期 页码：1280.

关键词：粉防己碱；获得性多药耐药；小鼠；P170；LRP；TOPOⅡ；

Keywords:tetrandrine, tumor, mouse, multi-drug resistance, P170, LRP, TOPO Ⅱ,

摘 要 ：目的:观察粉防己碱口服给药对化疗诱导的多药耐药基因表达产物P₁₇₀、肺耐药蛋白LRP和拓扑异构酶Ⅱ(TOPOⅡ)的影响。方法:以亚于治疗剂量的联合化疗PFC方案,化疗诱导建立小鼠S180肿瘤细胞多药耐药模型,同时给予粉防己碱4周,流式细胞仪荧光检测P₁₇₀,LRP,TOPOⅡ。结果:粉防己碱明显降低化疗诱导后耐药细胞P₁₇₀,LRP的表达率和ROPOⅡ活性。结论:粉防己碱可通过抑制耐药基因表达和DNA拓扑异构酶活性,干预化疗诱发的肿瘤细胞多药耐药的产生。

Abstract:Objective: To observe the effect of tetrandrine on the P₁₇₀ production expressed by multi-drug resistance gene, lung resistant protein (LRP), and topoisomeras Ⅱ and elucidate the underlying molecular mechanism. Method: Cellular model of multi-drug resistance was established in S180 tumor cell by means of the scheme of PFC chemotherapy at the dosage lower than that with curative effect. P₁₇₀, LRP and TOPO Ⅱ were measured by flow cytometry after the mouse model was treated with tetrandrine for 4 weeks. Result: tetrandrine obviously reduced the enhancement of express of P₁₇₀, LRP and the activity of TOPOⅡin the tumor cells with multi-drug resistance induced by chemotherapy. Conclusion: Tetrandrine significantly inhibits the multi-drug resistance of tumor cells induced by chemotherapy via diminishing both the expression of multi-drug resistance gene and the activity of topoisomeras Ⅱ.

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