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期 刊 ：中国中药杂志 1999年 24卷 04期 页码：205.

关键词：wild mountain ginseng；DNA；extraction method；

Keywords:wild mountain ginseng, DNA, extraction method,

摘 要 ：目的：为了从珍贵药材野山参的少量根须提取ＤＮＡ，同时尽量保持野山参形态的完整，特研究本方法。方法：我们改进了ＣＴＡＢ提取法，设计了一种简捷的ＤＮＡ制备方案。结果：从０．００１ｇ人参根须提取ＤＮＡ，得率达２２５０μｇ／ｇ。结论：ＲＡＰＤ扩增效果良好，可作其他药材微量组织提取ＤＮＡ的参考方法。

Abstract:Objective:To keep wild mountain ginseng,a rare medicinal remedy,as intact as possible when extracting DNA from it,a new way to extract DNA has been explored.Method:The CTAB method was improved and a simple and direct way was developed. Result: From 0.001g of dried samples of ginseng root DNA was obtained in an amount up to 2.25μg.Conclusion:The RAPD fingerpringting generated by this DNA is as good as that by CTAB method.This new method may also be used for extraction of other precious Chinese medicinal materials.

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