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期 刊 ：中国中药杂志 1998年 23卷 07期 页码：424.

关键词：草苁蓉；胎盘型谷胱甘肽S-转移酶(GST-P)；化学致癌作用；抑制效应；p53蛋白；

Keywords:Boschniakia rossica, placental form glutathione S transferase, chemical carcinogenesis, inhibitory effect, p53 protein,

摘 要 ：目的：探讨草苁蓉（ＢＲ）对大鼠肝脏化学致癌作用的抑制效应。方法：按Ｓｏｌｔ-Ｆａｒｂｅｒ氏方法复制大鼠肝脏癌前病变模型，用免疫组织化学方法观察了在肝脏癌前病变组和ＢＲ处理组及正常对照组大鼠肝脏中胎盘型谷胱甘肽Ｓ-转移酶（ＧＳＴ-Ｐ），突变型Ｐ５３蛋白及Ｐ２１蛋白的表达水平。结果：发现５００ｍｇ／ｋｇ剂量的ＢＲ甲醇提取物对二乙基亚硝胺（ＤＥＮ）诱发Ｆ３４４大鼠肝脏ＧＳＴ-Ｐ阳性灶的形成有抑制作用，而且其突变型Ｐ５３蛋白及ｒａｓ基因产物ｐ２１蛋白的表达水平显著低于癌前病变组，并在ＢＲ处理后，在肝脏化学致癌初期由于癌前病变的形成所增高的γ-ＧＴ活力明显下降。结论：提示ＢＲ对ＤＥＮ诱发大鼠肝脏癌前病变有抑制作用。

Abstract:Objective: To investigate the inhibitory effect of Boschniakia rossica(BR)on hepatocarcinogenesis in rats. Method:Based on immunohistochemistry techniques,the expression of placental form glutathione S-transferase(GST-P),mutant p53 and p21 protein were investigated in hepatic preneoplastic lesions induced by Solt-Farber protocol in the liver of rats that had been treated with the above method, administered with BR extract and of control groupResult:The extract of BR(500mg/kg)has inhibitory effect on the formation of diethylnitrosamine induced GST P positive foci in F344 rat and the expression of mutant p53 and p21 protein was lower than that of hepatic preneoplastic lesions, and the increasingr-glutamyltranspeptidase(r-GT)activity in rat liver treated with Solt Farber protocol was decreased by the extract of BR,Conclusion:These results indicate that BR has inhibitory effect on DEN induced hepatic preneoplastic lesions in F344 rat.

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