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期 刊 ：中国中药杂志 1998年 23卷 06期 页码：367.

关键词：赤芍801；没食子酸丙酯；4-羟基苯甲酸乙酯；高效液相色谱法；

Keywords:Chishao801, propyl gallate, 4-hydroxybenzoate, HPLC,

摘 要 ：用反相高效液相色谱法测定家兔血浆中赤芍801PG浓度。采用NucieoselC₁₈色谱柱,甲醇∶水∶冰乙酸(48∶50∶2)为流动相,4 羟基苯甲酸乙酯为内标物,紫外检测波长275nm,对ivPG后不同时间家兔血浆中药物含量进行测定。结果表明:PG浓度为2.5μg/ml的平均回收率为96.70%,其RSD=1.83%。浓度5μg/ml的样品平均回收率为98.86%,RSD=1.05%。其最低检测限为0.1μg/ml。

Abstract:A HPLC method was used to determine the content of Radix Paeoniae Rubar (Chishao)₈₀₁in rabbit‘s plasma, using Nucleosil C₁₈column, methanol, water and glacial acetic acid (48∶50∶2) as mobile phase ethye 4 hydroxybenzoate as internal standard,and UV detector(λmax∶275nm, AUFS∶0.02).The average recovery was 96.70% (C:2.5μg/ml) and 98.86%(C: 5μg/ml) respectively. The lowest determination limit was 0.1 μg/ml.The method is simple, accurate, sensitive and reliable.

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