Abstract:The contents of salidroside and lotaustralin in 10 species of Rhodiola genus were determined by GC method with propionylated mannitol as internal standard compound under the condition of silylation with hexamethyl disilazane:trimethyl chlorosiland (2∶1).The recoveries are 100.05% (salidroside) and 97.85% (lotaustralin).