Abstract:Thirteen alkaloids were isolated from the seeds of Strychnos nux-vomica. They were identified as strychnine, β-colubrine, pseudostrychnine, strychnnine N-oxide, brucime, brucine N-oxide, novacine,icajine, vomicine, isostrychnine, isobrucine,isobrucine N-oxide and isostrychnine N-oxide by chemical and spectroscopic analysis.