Abstract:Based on field investigation and identification of 19 speies and one variety of medicinal plant of.Berberis produced in Guizhou Province ,it has been found out that among these species 3 is found to be new ,4 speciesand one variety are discoyered for the first time in Guizhou Province. Geographical distribution ,perpendicular distribu-tion and medicinal uses of these species are spelled out and a key for their identification is given.