Abstract:A TLC-donsitometrie method for the determination of arecoline in Semen Areeae(Areca eatechu) was established, and arecoline content in three Semen Arcane (Imported, Hainan, Guanzhou) was determined. The method is accurste, sensitive and simple. The recovery is 102.39% and the coefficient of variation of six asmplings is 1.95%. The are coline average content in the three Semen Arecae is 0.22%, 0.19%, 0.56% respectively.