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期 刊 ：中国中药杂志 1994年 19卷 09期 页码：551.

关键词：HPLC芫花；芫花酯甲；含量测定；

Keywords:HPLC, Daphne genkwa, yuanhuadne, content determination,

摘 要 ：采用Lichrosorb5RP18柱,以甲醇-水(82:18)为流动相,检测波长为232nm,进样量在7.6×1O￣-3～0.76μg间线性良好,加样回收率为99.56%,RSD为3.61%(n=9)。测得其含量为0.00848%。

Abstract:Separated on phenox RP18 column with a mobile phase of methyl alcohol-water(82 : 18) and detected at wave-length 232nm ,yuanhuacine has shown a good linearity in the range from 7.6× 10^-3 to 0.76μg. The recovery is 99.56%with RSD 3.61 %(n=9). The content of yuanhuacine in Daphne genkwa is 0. 00848%

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