Abstract:A study was made on the effects of Epimedium sagittatum polysaccharides on DNA synthesis of hone marrow cells of "Yang Deficiency" animal model caused by hydroxyurea with methods of cell culture in vitro and determination of 3H-TdR incorporating DNA of hone marrow cells. The results showed that after the treatment with 100μg of Epimedium sagittatum polysaccharides, the cell multiplication rate was increased by 72% and DNA synthesis rate increased by 68%, in 1×106 cells.