Abstract:Objective To study the effect of anti-HIV-1 virus with extracts from the tuber of Typhonium giganteum,so as to discover new and high efficient anti-HIV-1 leading compounds from natural products and Chinese materia medica.Methods Extract from the tuber of T.giganteum was isolated and purified by the way of phytochemistry,such as column chromatography.Then anti-HIV-1 activities of the extracts were assessed by in vitro method,MT4 cells and HIVⅢB virus were used for the experiment.The results were judged by cytopathic effect(CPE) method and p24 antigen assay method.Mechanism studies were carried out by BIA techniques(BIACORE~(?) 3000 molecule coupled equipment).Results The extracts of T.giganteum showed potential anti-HIV-1 activities.Two of them showed gp-41 transmembrance protein and Vif protein expression inhibition,respectively.Conclusion These extracts in the tuber of T.giganteum might rewardingly contribute to anti-HIV-1 activities,which could be developed to be more efficient and less toxic leading compounds in the further.