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作 者 ：岳春华,李顺祥

期 刊 ：中草药 2008年

关键词：骨碎补；新北美圣草苷；柚皮苷；对照品；

Keywords:Drynaria fortunei (Kunze ) J. Sm., neoeriocitrin, naringin, reference substance,

摘 要 ：目的建立从骨碎补中制备新北美圣草苷和柚皮苷对照品的方法。方法结合大孔树脂富集、硅胶柱色谱、中性氯化铝吸附、Sephadex LH-20柱色谱和重结晶方法对骨碎补乙醇提取物进行分离纯化,通过MS、IR、UV、¹H-NMR和¹³C-NMR进行结构鉴定。结果制备得到的新北美圣草苷和柚皮苷质量分数分别为99.5%、99.3%。结论建立的制备方法>简单,对照品质量分数高,可作为骨碎补药效物质基础研究和骨碎补药材质量控制的对照品。

Abstract:Objective To establish a separation method for neoeriocitrin and naringin reference sub- stances from Drynaria fortunei.Methods The extract of D.fortunei was isolated and purified by macro porous resin,silica gel column chromatography,neutro-aluminum oxide adsorbing,Sephadex LH-20 co- lumn chromatography,and recrystallizations.The structures of neoeriocitrin and naringin were identified by MS,IR,UV,¹H-NMR,and ⁽¹³⁾C-NMR.Results The contents of neoeriocitrin and naringin were 99.5% and 99.3%,respectively.Conclusion The developed method is simple with lower cost,by which neoeriocitrin and naringin can be used as reference substances for the qualitative and quantitative analysis of Chinese herbal medicine.

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