全 文 ：收稿日期:2008-08-01; 修订日期:2009-02-10
基金项目:广西教育厅科研基金(No.200501066)
作者简介:李勇文(1969-),男(汉族),广西柳州人 ,现任桂林医学院副教
授 ,硕士学位 ,主要从事中药药理研究及教学工作.
StudyontheAntihyperlipidemiaEfectofBuxusmicrophylaLeafEx-tract
LIYong-wen,YANGCheng-fang, ZHANGHui-qin, LILi
(DepartmentofPharmacology, GuilinMedicalUniversity, Guilin, Guangxi541004, China)
Abstract:ObjectiveTostudytheantihyperlipidemiaefectsofdiferentBuxusmicrophyllaleafextracts.MethodsDiferentextracts
wereextractedfromBuxusmicrophylaleafbysystematicsolvents(petroleumbenzin, ethylacetate, chloroform, n-butylalcoholand
waterrespectively)andtheantihyperlipidemiaeffectsofdifferentextractswereobservedonserumlevelsoftotalcholesterolandtri-
glycerideinhyperlipidemiamodels, whichwereestablishedthroughceliacinjectionof75% egg-yolkemulsion.ResultsTheBuxus
microphyllaleafethylacetateextract(highdoseandlowdose)hadobviouseffectonthelevelsofserumtotalcholesterolandtriglyc-
eride.Moreover, theeffectofhigh-doseethylacetateextractwasbetterthantheotherextracts.Inaddition, thewaterextractandn
-butylalcoholextractcouldsignificantlyreduceserumtotalcholesterol, andthechloroformextractandn-butylalcoholextracthad
noremarkableefectonbloodlipid.ConclusionBuxusmicrophylaleafethyl-acetateextractistheactivepartofBuxusmicrophyl-
laleafinreducingbloodlipid.
Keywords:Buxusmicrophylaleaf; Extracts; Antihyperlipidemiaeffect
Clcnumber:R285.5 Documentcode:A ArticleID:1008-0805(2009)08-1918-02
BuxusMicrophylaleafisthedryleaffromBuxusmicrophyla.
(FamilyGenus).Accordingtothe“CompendiumofMateriaMedi-
ca”, Buxusmicrophylacanpromoteflowof“qi” andbloodcircula-
tion, eliminatepathogenicwetness, dredgeandactivatethemeridi-
an.CyclovirobuxineDisonekindofalkaloidextractedfromtherhizo-
meofBuxusMicrophyllaandotherplantsbelongedtothesameGe-
nus[ 1] .Inrecentyears, clinicalyandexperimentalyCyclovirobuxine
Dplaysagoodroleinanti-arrythmia, anti-myocardialIschemia
andstrengtheningheart, andithasprotectivefunctiontotheacute
brainischemiabecauseitcanpassthroughthebloodbrainbarrier,
suppresstheformationofthrombus, improvemicrocycle[ 2-3] , howev-
er, untilnownopharmacologicalresearchreportsontheBuxusMicro-
phyllaleafhasbeenseenintheliterature.Inthisexperimentweob-
servedtheefectsofsystemsolventextractfrombuxusmicrophylla
leafontheserumtotalcholesterolandtriglycerideinhyperlipidemia
modelmice.
1 MaterialsandMethods
1.1 Drugs, Reagents, AnimalsandInstruments
1.1.1 PreparationofdrugsDryBuxusmicrophylaleaves, from
Yongfu, GuangxiGuilin, wereextractedwith70percentethanol.The
processwasasfollows:Firstly, 500gBuxusmicrophylaleaveswere
takenintoroundflask, atthesametimeadded3 000 ml70% ethyl
alcohol, thenheatedbackflow, totallythreetimes, threehourseach
time.Afterextractingthefiltration, mergedthefiltrate.Finnallythe
grossextractswereconcentratedtonon-alcoholflavor.Thegross
extractswereextractedwithbenzine, ethylacetate, chloroform, nor-
malbutylalcoholrespectivelyaccordingtoresolverpolarityorderfrom
smaltobig, theneveryextractedliquidwasevaporatedonthewater
bathtogeteachvariouspartofextractions(aftermultiplewithdraws,
obtainsstablefingerprintatlas).Whenexperimentbeingcarriedon,
eachextractionwasdissolvedwith2% Twain- 80 distiledwater.
1.1.2 ReagentsXueZhiKangcapsule(BeijingUniversity, WBL
BiotechCo, LTD.Batchnumber:20040513);
Totalcholesterolreagentkit(ZheJiangDongOuBiologicalEn-
gineeringCo, LTD.Batchnumber:2006120060);
Triglyceridedeterminationreagentkit(ZheJiangDongOuBio-
logicalEngineeringCo, LTD.Batchnumber:2006120173);otherre-
agents, analyticalreagentavailableonmarket.
1.1.3 AnimalsOnehundredandthirtySPF-Kunmingmice, 8-
10 weeks, weighing(25.0±2.0)g, maleandfemale, suppliedbyex-
perimentalanimalcenterofGuangximedicalcollege(CertificateNo:
GuiDongacceptanceproposal2003 -0003st);Alanimalexperi-
mentswerecariedoutaccordingtotheguidelinesforthecareand
useofthelaboratoryanimalsandwereapprovedbytheExperimental
AnimalEthicalCommitteeofGuilinMedicalUniversity.Alpossible
effortsweretakenduringtheseexperimentstoavoidunnecessarysuf-
feringandtominimizethenumberofanimalsused.
1.1.4 Instruments 722 -modelUV-visiblespectrophotometer
(ShanghaiPrecision＆ScientificInstrumentCo., LTD);TGL-168
high-speeddesktopcentrifuges(ShanghaiAntingScientificInstru-
mentFactory);Digitaltwoheatedwaterbathpot(ZhengzhouGreat-
walScientificIndustrial＆TradeCo., Ltd).
1.2 Methods
1.2.1 Divisionandadministration Onehundredandthirtymice,
maleandfemale, weight(25.0 ± 2.0)g, wererandomlydivided
into13 groups, eachwith10, namely:⑴controlgroup;⑵model
group;⑶XueZhiKanggroup;⑷Waterextracthigh-dose;⑸Water
extractlow-dose;⑹Petroleumetherextracthigh-dose;⑺Petrole-
umetherextractlow-dose;⑻Ethylacetateextracthigh-dose;⑼
Ethylacetateextractlow-dose;⑽Chloroformextracthigh-dose;⑾
Chloroformextractlow-dose;⑿n-butylalcoholextracthigh-
dose;⒀n-butylalcoholextractlow-dose.Sixteenhoursafterfast-
ing, themiceofgroup(1)and(2)weregivensolvent(distiledwa-
terincluding2% Twain-80, 0.2ml/10 g)bygavage;themiceof
Group(3)weregivenXuezhikang(0.24 g/kg)byintragastricad-
ministration;Experimentalhigh-dosetreatmentgroupsweregavage
correspondingdrug0.90g/kg;low-dosegroupweregavagecore-
spondingdrug0.45 g/kg.Alabovegroupsdeliverycapacitieswere
0.2 ml/10 g.Twohourslater, exceptblankgroupaltheothermice
wereinjectedintraperitonealywith75 percenteggyolkemulsion,
dose0.1ml/10g(75percenteggyolkemulsion:eggyolkfrom1-
2 dfresheggsmatchedwithsaline).Sixhourslater, eachgroupwas
administeredbygastricperfusioncorrespondingsolventorthemedi-
cineoncemore, thedosagedetto, twentyhoursafterinjectionofegg
-yolkemulsion, bloodwasextractedfromtheophthalmicvenous
plexus, thenbloodserumwasseparated(3 000 RPMperminute,
centrifugal15minutes).Finaly, serumtotalcholesterol(TC)and
triglyceridecontent(TG)weremeasured[ 6, 7, 8] .
·1918·
时珍国医国药 2009年第 20卷第 8期 LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2009VOL.20NO.8
1.2.2 DeterminationTotalcholesterolwasdeterminedbyCHOD-
PAP;triglycerideswasdeterminedbyGPO-PAPlawoperatedac-
cordingtospecification.
1.2.3 StatisticaltreatmentThemeasurementdatawereexpressed
asmean±standarddeviation( x±s)andanalyzedbyANOVAwith
SPSS1 0.0 software.
2 Result
Serumtotalcholesterolandtriglyceridelevelsofthemodelgroup
werehigherthanthoseinthecontrolgroup, thediferencesweresta-
tisticalysignificant(P<0.01), hyperlipidemiamodelsuccessed.In
contrastwiththemodelgroup, thelevelsofserumtotalcholesterolin
miceofwaterextractsofhigh-andlow-dosegroupsandpetroleum
etherextractsofhigh-andlow-dosegroupsandethylacetateex-
tractsofhigh-andlow-dosegroupswerelower, thedifferenceswere
statisticalysignificant(P<0.01).Comparedwiththemodel, the
levelsoftriglycerideinmiceofethylacetateextractsofhigh-andlow
-dosegroupswerelowerthanthatofmodelgroups, thedifferences
werestatisticallysignificant(P<0.01), butthewaterextractsof
high-andlow-dosegroupsandpetroleumetherextracthigh-and
low-dosegrouphadnostatisticallydiference(P> 0.05).Com-
paredwiththemodelgroup, thecontentsoftotalcholesterolandtri-
glycerideschloroformextractsofhigh-andlow-dosegroupsandn
-butylalcoholextractsofhigh-andlow-dosegroupshadnosignif-
icantstatisticaldifference(P>0.05).Seetable1.
3 Discussion
Hyperlipidemiaresultedfromlipidmetabolismdisorderinthe
bodyisacommondiseaseintheelderly, frequently-occurringdis-
ease, anditisariskfactorleadingtoarterioscleroticcardiovascular
disease, seriouslydoesharmtohumanhealth.Withtheimprovement
oflivingstandards, thepopulationofhyperlipidemiaisincreasing,
thedemandofhumantohypolipidemicdrugsisongrowing, hence,
thedevelopmentofhypolipidemicagentshavegoodsocialandeco-
nomicbenefits[ 9 , 10] .Aconsiderableamountofresearchshowsthatal-
kaloidsisolatedfromwoodofBuxusmicrophyla(suchasCycloviro-
buxineD)haveabeterclinicaleffectoncardiovasculardisease.
However, thestudyontheBuxusmicrophylaleafisless, andthe
studyonitshypolipidemicisntmorereportedinliteratures.Inthis
studythesystemsolventwasusedtogetdiferentextracts.Atthe
sametimetheirefectsonmicehyperlipidemiawereobserved.The
resultsshowthatethylacetateextractsfromBuxusmicrophylaleaf
(0.45-0.90g/kg)significantlyreduceleveloftheserumtotalcho-
lesterolandtriglycerideinhyperlipidemiamice.Itprovidestheexper-
imentaldataforfurtherstudyonhyperlipidemicactiveingredientsand
exploreGuangxisrichplantresources.
Table1 TheefectsofthediferentextractsfromBuxusmicrophylla
leafonTCandTGinhyperlipidemiamodelmice. x±s, n=10
Groups DoseC/g· kg-1· d-1
TC
mmol/L
TG
mmol/L
⑴controlgroup 2.25±0.84＊ 1.05±0.26＊⑵modelgroup 6.71±1.34# 3.96±0.54⑶Xzkgroup 0.24 4.17±1.13＊ 1.23±0.46＊⑷Waterextracthigh-dose 0.90 3.26±1.62＊ 3.70±1.14
⑸Waterextractlow-dose 0.45 3.24±1.45＊ 2.50±0.63⑹Petroleumetherextracthigh-dose 0.90 2.12±1.35＊ 3.89±1.20⑺Petroleumetherextractlow-dose 0.45 3.93±0.86＊ 3.89±1.20⑻Ethylacetateextracthigh-dose 0.90 2.60±1.42＊ 0.91±0.40＊
⑼Ethylacetateextractlow-dose 0.45 3.46±1.51＊ 1.26±0.49＊⑽Chloroformextracthigh-dose 0.90 7.12±1.34
⑾Chloroformextractlow-dose 3.21±0.34 8.13±0.43 2.85±0.51
⑿n-butylalcoholextracthigh-dose 0.90 7.31±1.64 3.76±0.32
⒀n-butylalcoholextractlow-dose 0.45 6.33±1.60 3.22±0.39
References:
[ 1 ] ZhangKaiYuan, WuYiYuan.Thereviewofthehuangyangningclini-
caluse[ J] .primaryjournalofchinesemateriamedica, 2000, 14(4):
52.
[ 2 ] LiuRuYing.Theclinicuseofthehuangyangningtablet[J].Journalof
clinicalinternalmedicine, 1997, 14(2):112.
[ 3 ] TaoXinYietal.ProtectiveefectsofCVB-Donthecerebralischemia
injury[ J] .Pharmacologyandclinicsofchinesemateriamedica, 2003,
19(5):13.
[ 4 ] ZhaoYongMei, LlFaRong, YANGJIANXIONG, etalStudyonthe
reducingbloodlipidandantioxiditioneffectofphilyrin[ J] .Nature
productionresearchanddevelopment2005, 17(2):157.
[ 5 ] ZhangRong, FengMaLl, WuYuPeng, etal.Experimentalstudyonthe
activesitusoffetidcassiaseedtoreducebloodlipidandtheirdose-
efectrelation[ J].Chineseremediesandclinics, 2005, 5(3):183.
[ 6 ] ChenQi, Thestudymethodologyonthepharmacologyofthechinesetra-
ditionalmedicineandmaterialmedica.BeiJing:People, smedicalpub-
lishinghouse1993, 519.
[ 7 ] WangShiMin, YangYong, WuYuPeng, etal.Experimentalstudyon
efectofFangfengTongshengpiloncholeterol[ J] .Pharmacologyand
clinicsofchinesemateriamedica, 1989, 5(3):3.
[ 8 ] ZhouRuiLing, ChenYuXing, CengXiaoHui, etal.Experimental
StudyontheAntihyperlipidemiaEfectofFufangJiangzhiling[ J].China
JournalofExperimentalTraditionalMedicalFormulae, 2006, 12(6):
43.
[ 9 ] XieHuiPing, LiuYiNong, GuiMing, etal.ExperimentalStudyofthe
MulberyLeafExtract, sEfectonBloodLiqid[ J] .ModernMedicine
JournalofChina, 2006, 8(11):48.
[ 10] ZhangChunPing, LuiXuePing, HuangXingZheng, etal.Studyonthe
SerumLiqidandAnti-atherosclerosisEffectofCameliaoil[ J].Chi-
neseJournalofHospitalPharmacy, 2007, 27(5):596.
小叶黄杨叶降血脂作用研究
李勇文 ,杨成芳 ,张惠勤 ,李 丽
(桂林医学院 ,广西 桂林 541004)
摘要:目的 研究小叶黄杨叶不同提取物的降血脂作用。方法 选用系统溶媒(石油醚 、醋酸乙酯 、氯仿 、正丁醇 、水)的方
法对小叶黄杨叶进行提取 ,观察各提取物对腹腔注射 75%蛋黄乳液致高血脂症模型小鼠血清总胆固醇及甘油三酯的影
响。结果 小叶黄杨叶醋酸乙酯提取物的高 、低两个剂量组均有显著的降低血清总胆固醇和甘油三酯的作用 , 小叶黄杨
叶的水提取物和石油醚提取物均有显著的降低血清中总胆固醇的作用 , 小叶黄杨叶氯仿提取物及正丁醇提取物均无降
血脂的作用。结论 小叶黄杨叶醋酸乙酯提取物对高血脂症小鼠有降血脂作用。
关键词:小叶黄杨叶; 提取物; 降血脂
·1919·
LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2009VOL.20NO.8 时珍国医国药 2009年第 20卷第 8期