Determination of vaccarin in Vaccariae Semen by HPLC Isolation and identification of vaccarin from Yaccariae Semen Effects of Soil Moisture on Biomass of Vaccaria segetalis(Necr.) Gracke and Vaccarin Content Isolation and identification of vaccarin from Vaccariae Semen Establishment of culture system of Vaccaria segetalis hairy roots and determination of vaccarin