Selecting the Electrofusion Condition of Alfalfa Protoplast Maize Genetic Transformation Mediated by Agrobacterium tume faciens and Optimization of the Differentiation and Rhizogenesis Conditions Determination of Lipoxygenase Activity in Barley Grain by UV Spectrophotometry Protoplasts Culture and Fusion from Callus of Poa pratensis L. Extraction and enzymatic properties of β-glucosidase in leaves of Baphicacanthus cusia