免费文献传递   相关文献

长穗偃麦草y型高分子量谷蛋白基因的鉴定与分子克隆



全 文 :! !#$!%&’()*+,-./#$
012!3##$# 4($5678(%
!! 9:;< %&’()*+,)*+-)**./0)12.1-.3+456789:;<*6=>/8-7&3.2&3-1?3+@.9:ABC5C$BCD#E#
+++++=>?@:BAAD5AE5B#++++AB?@:BAAD5A$5AC
CD()*+EF F)&*179+),+%<*8-&9’&*79+G8)’.-(1)9)&78GH&
IJKLM HNOPQRSTU!VWXYZPQ[\ !
!# $%& ’() *+, !!
N%&CD-E]L78^$ _‘a MCDA’
-./bc OPO5Q%R4 de$fIJKLM( 49H’*8<87 .9)1<7’7$4.4.$B1TBUTE’ WOPQRSTUghi78% jIJK
LMkl QV#CWBM m$DD noQpq BXE rstuvWOPQRSTUw!$ x E ryz{|$ }~IJKLM€
QV#CWBM OPQRSTU‚ƒ„…†‡W% bc noQˆ PY% f‰TU!VŠ‹Œgh QZ[ Ž$ q B3E)B3)3C  3W+
\=+E ‘’$P“”=•[\– @5—˜„$™š›œn 04.B3E$04.B3$04.3W  04.3C%‰m$04.3W  04.3Cz H Nw
!$Z]Lž)o %)G)P  [ !VŸŠ‹W H NOPQRSTU!V P¡¢%}g£¤¥¦iIJKLM§qOPQRS
TU!V%
012/IJKLM*OPQRSTUw!*PQ[V2.1’8,8-7’8)1+712+])9.-&97*+Z9)181<+),+H5’H0.+^8<(56)9.-&97*5_.8<(’S
R9&’.181SO&=&18’SR.1./S,*)6 49H’*8<87 .9)1<7’7SN^)/’KSY.‘/\8
a%YRSF85[&8SSSSb%YSc.5^)1N@*8’8-.7.S[./.7*-(SV1/’8’&’.ISO8-(&71S%<*8-&9’&*79Se18‘.*/8’HISP&f871S@(.S(8<(56)9.-&97*5_.8<(’S<9&’.181S/&=&18’/S81S49H’*8<87 .9)1<7’7 N4.4.$B1TBUTEKS_.*.S7179Hg.2S=HSOPO5Q%R43S@(.S
49H’*8<87 .9)1<7’7S7--.//8)1SQV#CWBMS_7/S(.’.*)<.1.)&/3 S@_)S’)SES=712/S-)&92S=.S2.’.-’.2S81S28,,.*.1’S/..2/I S_(89.SES=712/S_.*.S
6)/’S-)66)13 S@(.SQZ[S76098,8-7’8)1S),S-)609.’.S)0.1S*.7281QZ[S,*76.S9.1<’(S_.*.S7=)&’SB3EI SB3I S3CS712S3WS\=I S*./0.-’8‘.9H3 S47-(S),S’(.6S_.*.S-)99.-’.2S712S-9)1.2S’)S@5‘.-’)*S712S,)&*S
<*)&0/S),S0)/8’8‘.S097/682N04.B3E$04.B3\=$04.3WS712S04.3CKS_.*.S)=’781.23S%6)1<.1./I S712S’(.8*S0*867*HS/’*&-’&*./SS_.*.S‘.*HS/86897*S’)S’(7’S),S0&=98/(.2S<.1./S)=’781.2S,*)6S%I SGI SPS712S[S<.1)6./S),S@*8’8-.7.
/0.-8./3 S V1S7228’8)1I S ’(8/S_)*\S_7/S ,&*’(.*S /&00)*’.2S ’(7’S49H’*8<87S /0.-8./S(72S ’(.S /86897*S(8<(56)9.-&97*5_.8<(’S<9&’.181S<.1./S
*.97’.2S’)S’(7’S),S_(.7’3
S49H’*8<87 .9)1<7’7 iS(8<(56)9.-&97*5_.8<(’S<9&’.181S/&=&18’iS6)9.-&97*S-9)181<
KLM¨N49H’*8<87SK…]Lž©4(Mª«)$
pq¬­®˜– ®˜W©o®N$ !ª¯°˜Ÿ
q!4=N4K$4.N4BK$O’NjK$^±kl% V‰²³›´µ$¶j
©o·¸r¹º(I$9»¼c½¾M% v¿$À¨Á
…ÂO]LÃRÄÅƜ›ÇW,È!VÉÊ!t
ËpqÌÍÎ)-LÏÐÎ)ÑÒΏUÓα©o
ÌÎ!V$Ôpq-J)©Õ)ÌÖ×ØÙÚÛ)ÌÜ)
ÌݱީßśÇkBm$l%àáKLMâZ{9]Lg
hãä†å$æçè‚é)êëé)ìíé)îPï­
®˜±ðWñèkl$Vò¼óô³cá]Lõo%
IJKLM…KLM¨W£­®˜)o$‰
¯°˜Ÿç 4.4.% d7_*.1-. ±kClö÷ÂøIJKL
MZ‰ù]Lž)o¡¢$ oQúûTUmpqO
PQRSTU$‰Š‹!V‚á 4¯°˜Iü% ý
þ$P‘)*7\k#l±ÿcIJKLM # ¯°˜P“êë
{9]LÆo+ m!, W %)G  P ¯°˜$
fêëéþêghSTUP#¥iKLMOPQ
RSTU!V‚á 4 ¯°˜Iü$ ‰TUPQR
! # $ % & ’ ( !#$$)
* %&$+,-./01 ’()*+!,$234536
789 -.-/’0125:
345$%&$-.-6’0127898:;<4<$=>7?@A75:BCD9497
72:;CF4548 D:=958CD78GGD<<4=97’()*+!,
%H)!%%H%*!!H!I7; !)HJ, <=>+,-.?01
’()*+!,723%!%)!!* ; JI >@.A2BCD2
3# %7C=7)K7%%7C=7%*L$!$C=$!I$89M$!)$C=$J,$8FD$$
2:;CF4548 D:=958CD$$8GGD<<4=9$’()*+!, %NO%)N$!*$89M$
JI$8FD$$$PQD8C$R8F4DC;$SQ49DEFG@.HIJ$KLMNOPQHRS%TUV
WX+,-.?4536789YZH[6% TNOB]^H-.?_‘aEbc@. ! -.?
def]^H-.?gh$ ijkl%U_FG@.
E-.?mnH@.oA2pqr -.-6’012 s
<5:%TUVtu-.?H4536789YZ$
vwxl V%%Uyz{>%+,-.?|VEFG@.
ghH4536789%^}~3; JW €H
‚$ƒ„H+,-.?4536789ZS…†‡
V %&J$XE% ˆE@.‰$2BŠ‹4536.78
9ZSHŒ536Ž@ %&)$XE ‘I’“”$
#$% &’()*+,-./012345678
9:;<=>?@ ABCDEFGHIJKLMN
OPQRSTUVWXYZ[\]^ _‘abcd
efghijklmnopqrstuvwx
!$$$01;•–
!!####!
—˜™+,-.? Y2:;CF4548 D:=958C8N $2D2DN $
!9Z![Z%#$\’()*+!,! ]-.060^-š›%RœMž
Ÿ$
!$#### $
!$!#OOO-.-6’0125:  ¡¢£lV%!UH•–pq$
!$$OOOO._0 š¤ ¥ %¦-.?23§¨©ª«
‘abcdefghi Sj0k –š¤ ._0$ |™¬
­  A89 lV%JUH•–pq$
!$%#OOO’S^ ƒ„ >rƒ„®-.?4536.
78¯ZSH°±²³%PQr ! _‚$&
’%&+W60j11Sj001S11Sljj0l11jSSjSjjj16JW
’ ( Slj) ´µ Sj ¶T* m
’!&+W6Sj0jS0Sj11Sj0l11SS10S00j1S16JWm
’J&+W601Sj1S010101jjSj0jS06JWm
’#&+W60jS0SSS0S00S0SS101S06JW$
·B%’%l’!O¸M²³¹%’Jl’#¸M²³º
no ’S^»¼½nE ’S^©ª  p89lV%#UH•–$
!$&OOOO’S^ …†¾¿+ ÀÁÂXE5: ¥ ’S^
qrstu Tk:BD-GF4TCO-vwxl6\j6 ÙB$ ÂÀL
ÄÅÆ ’ j8v8^8* pq% Ç^ _Sk( ÈÉBH
E:8yz{|}~
$OOOOÏÐ;5:
$!####%&’()*
5Ñ_-.?01 ’()*+!, H JJ ¦23pq
y -.-6’012 5:$ ÏÐtu%ˆ JJ¦23H8¯
©ÒÓÔÕÖgIh% 4536×®uH©Ò
z{ !|#%Ø[> # ©Y* %\$ ˆ´“+,-.?Ù
Ú ’()*+!,453678¯¸ÛÜÝÞßH% ]à
á23â>Þßãäå% æ453678¯ZS>
ç”è´é$
$$####+,-./
ê^ ’%l’! ‚$ƒ„%’S^ ƒ„œ$©ÒëìN
gíîïæ^ ’Jl’# ‚$pqƒ„ð%áñ # òó
ã胄…†% ·536ô> !&#!!&%!%&* ;
%&+OXE%õÂöB ! ò> [6 åYZ!÷ ! ò> ;6 å
€‚ZSø³ ƒ* !\$ 5ÑîïT¾¿ñ
Tk:BD-GF4TCO-vYxl6\j6 Ù%áñ #½ghHùè¾
„… †‡ˆ5ÑÂÀ%áñ +W; JWúûÀÁ$
$%####012)*
¥ # ½¾¿H +W ûE@.hü8¯ZSpq
ýþèÿ!$ ÏДµ% T2D%&+ ; T2D%&*’ ´ %*E
;å78¯ZSHýþè ’ 5ÑM )}; )#~*
â4M#$E ‰å78¯ZSHýþè ŽØ *+~
;ŽØ )~\%T2D!&% ; T2D!&# E [ å78¯ZS
Hýþèâ4M#$E Šå78¯ZSHýþè$
ˆ´“ ’()*+!, B|V ! ò [ å ’ T2D!&% ; T2D!&
#*; !ò ;å’ T2D%&+; T2D%&**YZZS$
%##
! ! #$%&#’()*+ $ ,-./0123456789./:;
! %&’(%)* &’(%)+# *,$%&’()*+,-./012
-./0(1%23(4565$1789&.:6;8418<1&’(%)*1.431&’(%)+1=65>+&?/06;>(3>6@>A980(7?0.:A=(6@>5@0?5(464;?/?465;64
*A5(:964.078364@:(@684
< !B’()*+ C2D+*!E =>/0?2
345@’ABC CFG DE
H6@B!B1CFG1.9&06<67.568418<1IJK1@0?5(4641
@(4(;1LGH;1<:891’0$5:6@6. (084@.5. 11111111
.77(;;6841C2D+*!E
%! MNO >/0FG !! CFG DE C2D+*!E ?
2345HBC# %B 1MNO119.:P(: !B 1CFG1
.9&06<67.56841 8<11 IJK1 @0?5(46411 @(4(;11 LGH;1
<:891’0$5:6@6. (084@.5.11.77(;;6841C2D+*!EB
!#$$$$3,45678
IJKLMNOPBQRST &’(%B*
Q &’(%B+ 6 F UVW N UV6X.Y4
Z[\! ]^_9 O$$R$D$M$%SW G$ 4
56 N$FV>‘abcd!ef\g< T#
h< T ij!&’(%B* W &’(%B+ klm
6?2345!_ N$F UVnop6Y4
Z.‘q %SW %Tr#stu9 M$%S 6 N
Vopvw% xk U r& !9 O$ 6 N Vo
VWX Y Z[\ %] W D r& # &’(%)* 9
R$D 6 F VY4Zopvw % n %T r& !
y9 M$%S W G$ 6 F Vopvz % xk
%*r& #&’(%)+9 O$6 FVopvw% n
T r& !y9 R$D 6 F Vopvz% k %%
^_ ‘abcde&’(%B*W &’(%B+q{rl
m6 $,-./0?2|HB45#
%}~
w * A ) * +  € O  ‚ ƒ $
fMfACOg’.„$ ./F…&†‡uˆ‰!
’()*+Šn->/0?2|!>/09
‹Kw*6Œ! HB45Žg %’€O
’h+i%%!%*j# ‘’“L fMfACOg’.„”’()*
kTT•–/6->/0?2|6—˜# ™Lš•’
()*+–/›œž6 MNOab CFGDE!Ÿ 
ST¡p¢£¤!:;]R[# ¥¦ZWQ§¨6Y
4Z[\m©¢ª«u›¬!&’(%B* W &’(%B+ k $
,-./0?2|45!]­nw* $,-./0?
2|456®,e %¯ !%rY4Z6°±²$%SUr
Y4Z6 NV$U!rY4Z6 FVWh³´µ²—
g(4R.4P[± ¶, ·© &’(%B* 9¸j¹4º»¢ lm&’(%B+ 9¸j¹4º»¢m
g(4R.4P.77(;;6844?9/(:-$&(f8?:7(; 23(4565$8<&’(%B*=65>&?/06;>(3;?/?46523(4565$8<&’(%B+=65>&?/06;>(3;?/?465;
nSTSU!111111111111111111111111O$111111111-:6567?9 .(;56o?91111111111111111111111111DS111111111111111111111111111111111111111111111111111111111111111111111111111DE
1111111111111nE%S!E11111111111111111111111 R$D1111111-B.(;56o?91111111111111111111111111111111111111D%111111111111111111111111111111111111111111111111111111111111111111111111111DU
1111111111111n%!D!D111111111111111111111111M$%S 1111-B.(;56o?91111111111111111111111111111111111111D!111111111111111111111111111111111111111111111111111111111111111111111111111D*
1111111111111OH!%E+ED11111111111111111111G$ 11111111f(7.0( 7(:(.0(11111111111111111111111111111111D%111111111111111111111111111111111111111111111111111111111111111111111111111D*
1111111111111J!!!S+11111111111111111111111Op!q 1111-B.(;56o?91111111111111111111111111111111111111+U111111111111111111111111111111111111111111111111111111111111111111111111111DS
1111111111111n%TD!]11111111111111111111111 Rp] 111111-B.(;56o?91111111111111111111111111111111111111+T111111111111111111111111111111111111111111111111111111111111111111111111111++
1111111111111n%!D!+11111111111111111111111 Rp]1111111-B.(;56o?91111111111111111111111111111111111111+*111111111111111111111111111111111111111111111111111111111111111111111111111DS
1111111111111OH!%E+E+ 111111111111111111Gp 1111111fB7(:(.0(1111111111111111111111111111111111111111+*111111111111111111111111111111111111111111111111111111111111111111111111111+E
< TB1&’(%B*$&’(%B+ 9 O$$R$D$M$%S$G$ 6 N V% .& $
F V% /& Y4Z[\¼d
H6@1TB1->(1789&.:6;8418<1&’(%B*1.431&’(%B+1=65>1U1&?/06;>(31$A5$&(1
>6@>A980(7?0.:A=(6@>51@0?5(464;16415(:9;18<1.96481.7631;(r?(47(1;6960.:65$1
641N1s.t1.431F1s/tA5(:964.01:(@684
O$$R$D$M$%S W G$½ g(4R.4P1 6[±>‘k nSTSU!u1nE%S!Eu1n%!D!D W
OH!%E+ED# z¾¿ÀKÁ¨¬6Y4ZÂ4!w¾¿À›ÃĂ6Y4ZÂ4#
->(11.77(;;684114?9/(:18<1O$$R$D$M$%S 1.431G$ 641g(4R.4P1=(:(1nSTSU!u1
nE%S!Eu1n%!D!D1.431OH!%E+EDu:(;&(756o(0$B->(17899841.96481.7631:(;63?(;1.:(1
;>8=(31/$1/6@1<:.9(u1.4315>(1;9.001<:.9(;1;>8=15>(1;?/;565?56841.96481.7631(;63?(;B
%U*
! # $ % & ’ ( !#$$)
*+,-.!/0123456789:;<=>1
+?@ABC6 %&’%’&! DEFGHI56J
KLMNOPQ3R$STMUVWXY23Z[
+456789:\]#
OPQ3R ()*+,-^ ()*+,._‘ /a\Wbc
d+456789:a\>1efgh# ()*+,-^
()*+,.+ijklmnt456789:a\uv p 0$1wxyWpq
a\zV{|+gh}~ ()*+,-€1@ABC
2 %&’%’& ‚ƒ 34 „…ijk† 1%51 ‡ˆ %1
‰ŠC+‹\kz†YŒ+‹k% ’67’!‡ˆŽ
‹k% 8768!  0 ywx‘+‹\k’\l
+-‘9“YŒ”•:0ywx‘ˆ +#‘‹\k–\;
—˜™‘8‹š›% &6<=! 1‹\k+‡hl‚z
{|—œpqa\# †œœžCŸ ¡^¢£>1
DEgh¤¥¦žC‘§+„…¨©+ >+5>!ª$
«¬ >1?­®¯€°±+²\l³—´€µe¶
+·G  >@5>#ª$+¨©¸œžC‘/¹
º»N€°±‘¼½/´µu¶+­®#
m¾¿ 0^ 1 wxy„…«¬NÀÁ^5
ÂÃĞC‘+„…ÀÁÅ1«¬;#DE\]+
ÆÇ^ÈÉÊ1ijk„…ËÌÀÁÃÍΫ¬u
ÏÐÑÒ+5Â#
! # $
+A$BC1IL<*O*A RI*7K, A S*LTLMCW(7!AAX7OM7VAL@AAX776, A JKP[L*OA CLcMML[*1IL<*O*ARLKIA)#AAf7A‘Fa^ãäå_GAHICPA?Fg^æçè_GABC76, A1INCWCOCW76A6CM7KLCNPOKAQNCWA)6/KNLDL7 *6C+444##UAd-hd4% L-AAHI7N*OLOK7V7MiDNCP<[A V/AWC6*MP67NAW7Ni*NO, A JMLA1IL<7A ^J*NL*OA1_G A
+444GA#!UAdd@jdd.A
dAAZLAEF‘ ^êÔë_G AZkPA‘FH ^ìí;_G AZLAS ^êî _G A*KA76,
1INCWCOCW76A 6CM7KLCKCA (ICO(ICNPOA [*QLML*&*1IL<*O*ARLKIA)\AAHI7*KA76, A’AOKP[/ACRI*7KAb7NL*K/A KINCPDIAMNCOO*OV*KR**K/(*O7<[A%NLKLMPW 7*OKLbPW,A’MK7ASCK,ASCN*76FcMML[*^ßùá$’â_GA+444GA+4U!#m!.^L7VOKN7MK_
.AA Z7RN*MC’((6A&*<*KGA+4.+GA-4U!-n@+
4AABbCN7iA ‘9 Ao7O7N[7ABAB9 ABL*K6*NAfAB9 A *KA 76, A1INCWCOCW76A
6CM7KLC
)6/KNPDL7 *6C+AHI776, A&*<*KLMA7<76/OLOACQAVLCMI*WLM76AQLCQA RI*7KFRI*7KDN7OO ^HICL’&)A7<[A’F>’&),A07KAJMLA‘Ag7NVLqILb^#î$%f’&’é’’â_9A+44.9A+#^+_UA.#r4
++AY7CQA gfXF&J+#A 7<[A +-A L
SCN*76LFACMML[*^L+!AE7*KA76, Ak[*OPVPuvwxyz_!++4 ^@_&+4\{+44 ^L)+@AX7A ?9 A *KA 76, A kOC67KLCMI7N7MK*NLt7KLCCQA D6PK*M/6L<[NLM76,A%I*CNA’((6A&*<*K9!!9+#U.!.|.@4
+#AE7D6PK*RLKIAKICO*ACQAICWC6CDCPOAOPVP#\U!!~!!-
+-$%776,$)(N*OOLCCQ$ ILDIFWC6*MP67NFR*LDIK$ D6PK*%ILSLCK*MI1IL<*O*$RLKI$)+#d