全 文 ：VariationofGlucoseContentinTartaryBuckwheat
Seeds
SHIZheng, SONGYu-xue, HANCheng-hua, HUANGKai-feng＊
InstituteofPlantGeneticsandBreeding, SchoolofLifeScience, GuizhouNormalUniversity, Guiyang550001
Abstract　[ Objective] Thisstudyaimedtofindingoutthediferenceinglucosecontentoftartarybuckwheatseedsfromdiferentorigins.[ Meth-
od] Taking35kindsoftartarybuckwheatfromdiferentoriginsasthetestmaterials, theglucosecontentofseedshadbeendetermined.[ Re-sult] Thevariationrangeofglucosecontentin35kindsoftartarybuckwheatwasfrom0.055 6% to0.840 2%withanaverageof0.321 7%.
Therewasadiferenceinglucosecontentoftartarybuckwheatfromdiferentorigins.TheseedsT324fromWeininginGuizhouhadthehighest
glucosecontent, whiletheseedsT398alsofromWeininginGuizhouhadthelowestglucosecontent.[ Conclusion] Thisstudyprovidesarefer-
enceforthegeneticvariationlawofglucosecontentindiferenttartarybuckwheatresources.
Keywords　Tartarybuckwheat;Glucosecontent;Geneticvariation
Received:January26, 2011　　Accepted:March14, 2011
SupportedbyScienceandTechnologyFoundationinGuizhou
[ GuizhouHeJ(2009)No.2108] ;KeyScientificResearchProject
forStudentsinGuizhouNormalUniversity(2010);ScientificRe-
searchFundforPhDinGuizhouNormalUniversity(2008).
＊Correspondingauthor.E-mail:hkfl979@163.com
　　Tartarybuckwheat(Fagopyrumtataritum)belongstocul-
tivatedcropsofbuckwheatgenus, polygonaceaefamily,
whichisknownasitsscientificnameofkangrabuckwheat[1] .
Accordingtoitscharacteristictraits, itcanbeclassifiedtotwo
categoriesincludingsweetbuckwheatandtartarybuckwheat.
Tartarybuckwheathaslightflavorwithslightbitterness, butits
nutritionalvalueismuchhigherthanthatofsweetbuck-
wheat[ 2] .Asthebirthplaceoftartarybuckwheat, Chinaisone
ofthemainproductioncountriesintheworld.Asakindofim-
portantcrops, tartarybuckwheathasmanyadvantagesthat
othercropscannotcompare.Moststudiesaremainlyaimed
toitsflavonoid, highly-activeproteinanddietaryfiber[3] , but
thereisrarestudyonglucosecontentoftartarybuckwheat.
Glucose, knownasbloodglucose, cornglucose, maizeglu-
coseorevenshortforglucose, isakindofquiteimportant
monosaccharidewidelydistributedinnaturalworld[ 4] .Glucose
isoneofthemaincaloriesources, and1 gofglucosecan
produce4 kcal(16.7 kJ)ofthermalenergy, soitcanbe
usedasthesupplementofthermalenergytocurehypoglyce-
mia, hypoalimentationcausedbytotalparentalalimentation,
starvationketosisandcomplementationoralargeamountof
humorallosssuchasvomitanddiarrhea.Accordingly, with
35 kindsoftartarybuckwheatfrom differentoriginsinthe
samecultivationasthetestmaterials, theglucosecontentof
seedswasdeterminedinthisstudytodiscussthegeneticvari-
ationlawofglucosecontentindiferenttartarybuckwheatre-
sources, whichaimedtoprovidingatheoreticalbasisforthe
comprehensiveutilizationoftartarybuckwheat.
MaterialsandMethods
Materials　
35 kindsoftartarybuckwheatwerefromInstituteofPlant
GeneticsandBreeding, SchoolofLifeScience, GuizhouNor-
malUniversity.AlmaterialsweresowninBaiyangtestsite,
InstituteofPlantGeneticsandBreeding, SchoolofLifeSci-
ence, GuizhouNormalUniversity, Guizhou, Longdongbao,
onAugust20, 2010.Eachmaterialwasplantedinasub-dis-trictwithareaof2 m×5 m, rowspacingof40 cmandinter-
plantdistanceof10 cmfornormalfieldmanagement.Seeds
werecolectedonNovember30, 2010, andthenstoredinthe
icecabinetat-20 ℃afterseeddrying.
Table1　Name, origin, sourceandsymbolofbuckwheatinthisstudy
No. Cultivar Origin Symbol
1 Daku Weining, Guizhou T282
2 Pingbi Weining, Guizhou T284
3 Heiku Hezhang, Guizhou T292
4 DanxuanF01 Weining, Guizhou T301
5 Ludian Yunnan T305
6 DanxuanF02 Weining, Guizhou T309
7 Yimengku Weining, Guizhou T324
8 Baizhengku Weining, Guizhou T330
9 Ku37 Weining, Guizhou T340
10 Chuanku1 Sichuan T345
11 Saobai Weining, Guizhou T349
12 K013 Weining, Guizhou T352
13 Ciku Weining, Guizhou T364
14 86-36 Weining, Guizhou T365
15 Pujiangku Liupanshui, Guizhou T367
16 19-22 Weining, Guizhou T373
17 Wugeheiku Weining, Guizhou T374
18 KP005 Shaanxi T384
19 Qianwei1 Weining, Guizhou T388
20 Heiku6 Weining, Guizhou T395
21 Xibaiku Weining, Guizhou T398
22 Longku Weining, Guizhou T412
23 Shanku Hezhang, Guizhou T415
24 Caobaku Hezhang, Guizhou T416
25 Ku1 Hezhang, Guizhou T425
26 Zhuangku Hezhang, Guizhou T429
27 Dachongku2 Weining, Guizhou T441
28 Xueshanku Weining, Guizhou T444
29 Yilaku Weining, Guizhou T446
30 Weiku02-286 Weining, Guizhou T452
31 Xiku6-14 Shaanxi T453
32 Ping01-043 Gansu T460
33 Yunku67 Yunnan T463
34 Yunku2 Shaanxi T468
35 Nayongku Nayong T470
EconomicGropsandMedicinalPlantsAgriculturalScience＆Technology, 2011, 12(2):214-216Copyright 2011, InformationInstituteofHAAS.Alrightsreserved.
DOI :10.16175/j.cnki.1009-4229.2011.02.030
Methods　
Thematerialsstoredintheicecabinetat-20 ℃ deacti-
vatedenzymesfor15 mininthedryingovenat105 ℃, and
driedatconstanttemperature(60 ℃)untiltoconstant
weight.Aftersheling, samplesweregroundbythemil, and
thenputintodryerforfutureuse.Beforetesting, samples
weredrieduntiltoconstantweightinthedryingovenat60 ℃.
Thedeterminationofglucosecontentreferencedtothemeth-
odbyZHOUQi[ 5] .
Dataprocessing　
DatawereprocessedbyExcel2003 software, andthe
diferencesignificanceanalysisofdataconductedbySPSS
17.0 software.
ResultsandAnalysis
Comparisonofglucosecontentindifferentkindsoftarta-
rybuckwheat　
FromTable2, thevariationrangeofglucosecontentin35
kindsoftartarybuckwheatwasfrom0.055 6%to0.840 2%with
anaverageof0.321 7%.Especialy, T324 fromWeiningin
Guizhouhadthehighestglucosecontent, whileT398 alsofrom
WeininginGuizhouhadthelowestglucosecontent.According
toglucosecontentofseeds(Table3), 35 kindsoftartarybuck-
wheatcouldbeclassifiedintothreetypes:low-glucosetypewith
15 kindsofglucosecontentbelow0.212 5%, accountingfor
42.86%ofthetestingmaterials;medium-glucosetypewith18
kindsofglucosecontentfrom 0.212 5% to0.683 2%, ac-
countingfor51.43% ofthetestingmaterials;high-glucose
typewith2 kindsofglucosecontentover0.6832%, account-
ingfor5.71%ofthetestingmaterials.
Table2　Comparisonofglucosecontentindiferentkindsoftartary
buckwheat %
No. Glucosecontent No.
Glucose
content No.
Glucose
content
1 0.084 0 13 0.142 9 25 0.403 4
2 0.064 2 14 0.062 7 26 0.459 4
3 0.688 2 15 0.060 2 27 0.535 5
4 0.320 4 16 0.091 7 28 0.164 5
5 0.067 2 17 0.161 7 29 0.148 3
6 0.659 9 18 0.358 1 30 0.388 4
7 0.840 2 19 0.442 3 31 0.575 9
8 0.300 6 20 0.554 5 32 0.676 1
9 0.182 9 21 0.055 6 33 0.471 4
10 0.426 6 22 0.391 5 34 0.215 4
11 0.634 8 23 0.063 1 35 0.385 8
12 0.086 4 24 0.096 2
Table3　Frequencydistributionofglucosecontent
No. Interval∥% Groupmedian∥% Frequence Cumulativefrequence Frequency CumulativefrequencyCr
1 0.055 6-0.212 5 0.134 1 15 15 0.428 6 0.428 6
2 0.212 5-0.369 4 0.291 0 4 19 0.114 3 0.524 9
3 0.369 4-0.526 3 0.447 9 8 27 0.228 6 0.771 5
4 0.526 3-0.683 2 0.604 8 6 33 0.171 4 0.942 9
5 0.683 2-0.840 1 0.761 7 1 34 0.028 6 0.971 5
6 0.840 1-0.997 0 0.918 6 1 35 0.028 6 1.000 0
Comparison ofglucosecontentin tartary buckwheat
seedsfromdifferentorigins　FromTable4, therewasa
diferenceinglucosecontentoftartarybuckwheatfromdifer-
entorigins.Especialy, thehighestglucosecontentoftartary
buckwheatseedsfromWeininginGuizhouwas0.840 2%,
whilethelowestwas0.055 6%withaverageof0.300 6%.Fur-
thermore, thevariationcoeficientofglucosecontentintartary
buckwheatseedsfromShaanxiwasthelowestof47.40%, while
thatfromYunnanwasthelargestof106.10%, whichindicated
thatitsglucosecontentwasnotstable.
Table4　Glucosecontentintartarybuckwheatseedsfromdiferentoriginsanditsmultiplecomparisonresults
Origin Numberofsamples Glucosecontent∥%Minimum Maximum Mean CV∥%
Weining, Guizhou 21 0.055 6 0.840 2 0.300 6b 77.62
Hezhang, Guizhou 5 0.063 1 0.688 2 0.342 1b 76.73
Liupanshui, Guizhou 1 - - 0.060 2c -
Nayong, Guizhou 1 - - 0.385 8b -
Sichuan 1 - - 0.426 6b -
Shaanxi 3 0.214 5 0.575 9 0.383 1b 47.40
Gansu 1 - - 0.676 1a -
Yunnan 2 0.067 2 0.471 4 0.269 3b 106.10
Differentlowercasesaftervaluesinthesamecolumnmeansignificantdiferenceatthelevelof0.05.
Conclusions
Saccharideisthemajorenergy-supplyingmaterialforhu-
manbeings, andsaccharideinfoodincludesstarchwiththe
largestmolecularweightsuchaspolysaccharide, disaccharide
(maltoseandsucrose), monosaccharide(glucose, fructose
andgalactose)[ 6] .Studieshaverevealedthatthereisa
diferenceinglucosecontentofdifferenttartarybuckwheatre-
sources, andT324 fromWeininginGuizhouhasthehighest
glucosecontent, whichisworthtobefurthercomprehensive
utilizationinproduction.Thereisalsoadifferenceinglucose
contentoftartarybuckwheatseedsfromdifferentorigins, and
theaverageglucosecontentoftartarybuckwheatinGansuis
thehighest.Thevariationcoefficientofglucosecontentintar-
tarybuckwheatinShaanxiisthelowest, whilethatinYunnan
isthehighest.
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Responsibleeditor:LIJing-wei Responsibleproofreader:WUXiao-yan
苦荞种子中葡萄糖含量变异研究(摘要)
时 政 ,宋毓雪 ,韩承华 ,黄凯丰＊　(贵州师范大学生命科学学院植物遗传育种研究所 ,贵州贵阳 550001)
[目的 ]明确不同产地苦荞种子中葡萄糖含量的差异。
[方法 ]以不同产地的 35份苦荞资源为试材 ,测定了其籽粒的葡萄糖含量。
[结果 ] 35份苦荞资源的葡萄糖含量变化幅度为 0.055 6%～ 0.840 2%,平均值为 0.321 7%;不同产地苦荞的葡萄糖含量存在差异 ,以贵州威
宁的苦荞种子T324中葡萄糖含量最高 ,原产贵州威宁苦荞种子 T398最低。
[结论 ]为明确葡萄糖含量在不同苦荞资源间的遗传变异规律提供了参考。
关键词　苦荞;葡萄糖含量;遗传变异
基金项目　贵州省科学技术基金项目[黔科合J字(2009)2108号] ;贵州师范大学学生科研重点项目(2010);贵州师范大学博士科研基金项目(2008年)。
作者简介　时政(1983-),男 ,江苏徐州人,讲师 ,硕士 ,从事植物遗传育种研究 , E-mail:drshiz1002@hotmail.com。 ＊通讯作者 ,副教授 ,博士 ,硕士生
导师 ,从事营养保健研究 , E-mail:hkf1979@ 163.com。
收稿日期　 2011-01-26　　修回日期　 2011-03-14
(上接第 200页)
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Responsibleeditor:SONGPing Responsibleproofreader:WUXiao-yan
茶树 EST-SSRs在山茶科植物中的通用性研究(摘要)
程小毛 1 ,陈自兰1 ,王华 2＊　(1.西南林业大学园林学院 ,云南昆明 650224;2.湖北省农业科学院中药材研究所 ,湖北恩施 445000)
[目的 ]研究茶树EST-SSRs在山茶科植物中的通用性。
[方法 ]选用来源于茶树EST序列的 7对 EST-SSRs引物 ,对山茶科植物的 19个种进行了扩增。
[结果] 7对EST-SSRs引物中有 5对引物能对 19个供试品种进行有效扩增 ,通用性比率为 71.43%;扩增率最高的为云南山茶凤山茶、华东
山茶彩霞和云南山茶菊瓣 ,最低的为川滇莲蕊茶和大花杨桐;有效扩增的 5对引物对中有 4对引物在供试材料中表现出丰富且差异明显的
多态性。
[结论 ]从茶树基因组上开发的SSR引物在山茶科植物不同种属植物间有较高的通用性 ,可用于山茶科植物比较基因组研究和分析标记研究。
关键词　茶树;EST-SSR;通用性;山茶科植物
基金项目　“省部级重点学科 、省高校重点实验室及校实验室共享平台 ”项目资助;西南林业大学校级重点基金项目(110909)。
作者简介　程小毛(1979-),女 ,安徽安庆人 ,讲师 ,博士,从事园林植物分子生物学研究 , E-mail:xmcheng0103@gmail.com。 ＊通讯作者。
收稿日期　 2011-02-17　　修回日期　 2011-03-02
216 AgriculturalScience＆TechnologyVol.12, No.2, 2011