Bioactivity and chemical composition of essential oil from Zanthoxylum beecheyanum var. alatum leaves against Culex pipens quinquefasciatus Diptera: Culicidae).
摘 要 ：参照“农药登记卫生用杀虫剂的室内药效评价/驱避剂GB/T 17322.10—1998”，研究了一摸香鲜叶的水蒸汽蒸馏精油对致倦库蚊的驱避作用;参照“电热片蚊香的室内药效测定方法GB 13917.5—1992”，测试了该精油对致倦库蚊成蚊的熏杀效果;采用浸液法测试了其对致倦库蚊幼虫及蛹的毒杀活性;并用GC-MS测试分析了其化学成分.结果表明：一摸香叶精油在1.5 mg·cm-2剂量下对实验室饲养的未吸血致倦库蚊雌成蚊的驱避100%保护时间为(6.54±0.17) h，对成蚊的熏杀24 h LC50值为6.895 μg·cm-3，对成蚊的熏蒸KT50值为6.46 min (熏蒸剂量为11.850 μg·cm-3)，对Ⅳ龄期幼虫的24 h毒杀LC50值为119.020 μg·ml-1.精油中共鉴定出17种化合物，占该精油挥发性成分总量的97.4%.说明一摸香叶精油对致倦库蚊具有显著的驱避作用和极强的毒杀活性.
Abstract:The repellent activity of steam-distilled essential oil from Zanthoxlu m beecheyamum var. alatum fresh leaves against Culex pipens quinquefasciatus was evaluated according to the “Laboratory Efficacy Criterions of Public Hea lth Insecticides for Pesticide Registration-Repellent (GB/T 17322.10〖KG-*2〗-〖KG-*7〗1998)” w ith some modifications in test procedure, the fumigating insecticidal activity o f the essential oil to Cx. pipens quinquefasciatus adults was assessed by th e “Efficacy Test Methods of Public Health Insecticides for Pesticide Registrati on-Method of Laboratory Efficacy Test for Electric Mat (GB 139175〖KG-*2〗- 〖KG-*7〗1992)”, and the larvicidal and pupicidal activities of the essential o il against Cx. pipens quinquefasciatus were investigated by immersion method in laboratory. Besides, the chemical components of the essential oil were analy zed and determined by gas chromatography-mass spectrometry. At the dosage of 1. 5 mg·cm-2, the essential oil provided 100% protection against laboratory-rear ed female blood-starved individuals of Cx. pipens quinquefasciatus for （654±017） h. The 24 h LC50 value of the essential oil for Cx. pipens quinquefasciatus adults w as 6895 μg·cm-3 when the fumigating dosage was 11850 μg·cm-3, and the KT50 value of the essential oil for the adults was 646 min. The 24 h LC50 value of the essential oil for IV in star larvae was 119020 μg·ml-1. Seventeen compounds in the essential o il were identified, accounting for 974% of the total. This essential oil exh ibited high repellent activity and remarkable toxicity against Cx. pipens qu inquefasciatus.