Biological characteristics of Metarhizium anisopliae var.major and its virulence to white grubs
以报道较多的蛴螬病原真菌布氏白僵菌、绿僵菌小孢变种作参照菌，系统研究了新分离鉴定的绿僵菌大孢变种的生物学特性，测定了其在偏低的自然气温下对蛴螬的致病能力.结果表明，绿僵菌大孢变种与参照菌的培养要求接近，最适宜的温度为25 ℃.3种供试的培养基中,PPDA为最适宜的培养基，其次为SDAY.在偏低的温度环境下绿僵菌大孢变种对蛴螬的毒杀能力强于布氏白僵菌，土壤和喷雾处理后累计僵虫率分别达88.23%和76.47%,有很好的田间应用前景.
With Beauveria brongniartii and Metarhizium anisopliae var.anisopliae as the reference,this paper studied the biological characteristics and invading ability of Metarhizium anisopliae var.major,a new variety separated from white grubs.The results showed that the optimal culture condition of M.anisopliae var.major was similar with that of other entomopathogenic species,and the optimal temperature was 25 ℃.Among the three test media,PPDA was the most appropriate one,followed by SDAY and Czapek.M.anisopliaevar.major had a stronger virulence than B.brongniartii at lower temperature,with an accumulative mortality of white grubs being 88.23% and 76.47%,respectively,after treated with soil and through spraying,suggesting that M.anisopliae var.major had a good prospect to widely apply in field.
全 文 ：