全 文 ：云南大学学报 (自然科学版) 　2002 , 24 (1):66～ 69 CN 53-1045/N　ISSN 0258-7971
Journal of Yunnan University
不同生长调节剂提高香荚兰抗逆能力的研究
田育天 ,郑焕娣 ,陈善娜 ,张　艳 ,周恒苍 ,杨明挚 ,陈小兰
(云南大学　生物技术系 ,云南　昆明　650091)
摘要:研究了 6 种不同生长调节剂脱落酸(abscisic acid , ABA , 5μg/ L)、膨大剂(CPPU , 5μg/ L)、那氏试剂
(778 诱导剂 , 5×106μg/ L)、烯效唑(uniconazole , 10 μg/ L)、长效 BR-120(0.2μg/ L)、几丁质(chitin , 10 μg/ L)
喷施香荚兰植株对其抗逆能力的影响.结果表明:各种处理后叶片内超氧化物歧化酶(SOD)、过氧化物酶
(POD)活性提高和丙二醛(MDA)含量降低.其中 , 烯效唑的效果最佳 , 其次为 BR-120 , 其它调节剂也有一定
的作用效果.此外 , 还对 ABA 处理后随取样时间不同植株抗逆能力的变化作了初探.
关键词:生长调节剂;香荚兰;抗逆能力
中图分类号:Q949.718.43;S 573.9　　文献标识码:A　　文章编号:0258-7971(2002)01-0066-04
收稿日期:2001-03-20
　基金项目:云南省九五攻关项目(95A2-8).
　作者简介:田育天(1968-　),男 ,四川人 ,硕士生,主要从事植物激素生理及抗性生理的研究.
67第 1 期　　　　　　　　　　田育天等:不同生长调节剂提高香荚兰抗逆能力的研究
68 云南大学学报(自然科学版)　　　　　　　　　　　　　　　　第 24 卷
Studies on Different Grow th-regulators in Increasing
the Stress Resistance of Vanilla
TIAN Yu-tian , ZHENG Huan-di , CHEN Shan-na , ZHANG Yan ,
ZHOU Heng -cang , YANG Ming -zhi , CHEN Xiao-lan
(Depar tment o f Biotechnology , Yunnan University , Kunming 650091 , China)
Abstract:Six grow th-regulators abscisid acid(ABA , 5μg·L-1),CPPU(5 μg·L-1), 778(5×106μg·
L-1),uniconazole(10 μg·L-1), BR-120(0.2 μg·L-1), and chitin (10 μg·L-1)were sprayed on the
leaves of Vanilla fo r studying of their ef fect on st ress resistance of Vanilla.The results showed that all the
used grow th-regulators increased the activities of supero xide dismutase(SOD)and peroxidase (POD), and
decreased the content of malondialdehyde(MDA)in the leaves.Among these t reatments , uniconazole and BR
-120 had the best ef fect in order , the others had some ef fects too.Study w as also done to the change n stress
resistance ofVanilla along wi th the sampling t ime af ter ABA treatment.
Key words:g row th-regulators:Vanilla ;st ress resistance
　　(上接第 65页)
Cloning and Expression of Human Collagen Ⅳα1 Chain NC1 Domain
TANG Hui1 , YIN Bin2 , SUN Wan-chen2 , WU Cheng-jun3 , CHEN Shan-na1 , PENG Xiao-zhong2
(1.Department of Bio technology , Yunnan University , Kunming 650091 , China;
2.Na tional Laboratory of Medical Molecular Biology , Institute of Medical Biolog y , Chinese Academy of
Medical Sciences/ Peking Union of M iedical College , Beijing 100005 , China;
3.Biotechnology Research Institute , Yunnan Academy of Agricultural Sciences , Kunming 650233 , China)
Abstract:It has been found that many components of collagens such as Collagen Ⅹ Ⅴ , Ⅹ Ⅷ NC domain
can inhibit proliferation , mig ration of vessel endothelial cell as w ell as suppress tumor grow th and metastased.
Here a pair are designed if specif ic primers accordng to the uncleo tide sequence of human collagen Ⅳα1 chain
NC1 domain w hich repo rted , then total RNA was ex tracted from human placenta tissue and human collagen
Ⅳα1 chain NC1 domain w as amplified by RT -PCR.The result f rom nucleotide sequencing indicated that
there is single nucleo tide mutation f rom A to C at position 132 but deduced amino acid(Gly)is no t changed.
The human collagen Ⅳα1 chain NC1 domain was then cloned into prodaryotic expression vecto r pPROEXHTb
and overexpressed in E .coli BL21.
Key words:collagen Ⅳ;α1 chain NC1 domain;PT -PCR;expression
69第 1 期　　　　　　　　　　田育天等:不同生长调节剂提高香荚兰抗逆能力的研究