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作 者 ：李晓楼

期 刊 ：生物技术通报 2016年 32卷 5期 页码：172-179

关键词：噻吩磺隆；枯草芽孢杆菌；生物降解；微生物制剂；

Keywords:thifensulfuron-methyl, Bacillus subtilis, biodegradation, microbial preparation,

摘 要 ：从土壤中分离到一株能高效降解噻吩磺隆的细菌LXL-7，经鉴定LXL-7为枯草芽孢杆菌（Bacillus subtilis）。在含100 mg/L噻吩磺隆的培养基中，菌株LXL-7降解噻吩磺隆的72 h降解率可达99.6%以上。菌株LXL-7的最适pH为7.5，适宜温度为30-35℃，该菌还对噻吩磺隆和盐有很好的耐受性，至少能耐受400 mg/L的噻吩磺隆和4.0%的NaCl。另外，还通过在商品化的土壤微生物改良剂中添加菌株LXL-7开发了多功能微生物制剂，新制剂除原有功能外还增加了降解噻吩磺隆的新功能，故认为菌株LXL-7能够被用于噻吩磺隆污染的处理以及相关微生物制剂的开发。

Abstract:A strain LXL-7 isolated from soil highly degrades thifensulfuron-methyl，and was identified as Bacillus subtilis. The degradation rate by LXL-7 reached 99.6% in mineral salt medium of thifensulfuron-methyl（100 mg/L）after 72 h. The optimal pH value and temperature were 7.5 and 30-35℃. The strain tolerated well to thifensulfuron-methyl and salt，at least to 400 mg/L of thifensulfuron-methyl and 4.0% NaCl. In addition，adding strain LXL-7 in the commercial preparation for soil amendment，a new multiple-function microbial preparation was developed，and it had an additional new function of degrading thifensulfuron-methyl residue in soil except original functions. Therefore，the strain LXL-7 could be considered for bioremediation of thifensulfuron-methyl pollution and development of microbial preparation.

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