Abstract:HcSCL13 gene of the GRAS family from Halostachys caspica(GenBank accession number：KC68640)was amplified and ligated to the pGBKT7 vector by homologous recombination to construct the bait vector pGBKT7-HcSCL13, and then transformed into the competent yeast Y2HGold. Self-activation of recombinant bait vector in yeast two-hybrid system was measured. The results showed that the bait protein had not toxic to Y2HGold and could activate all the reporter genes. Therefore, we proposed HcSCL13 was a transcription factor with a transcriptional activation domain. The plant over-expression vector and subcellular localization expression vector were successfully constructed for further studying its biological function.