Abstract:It was to study the effect of biomass and resveratrol content of peanut hairy root strains in different media. To induce hairy roots by using A.rhizogenes R1601 infect peanut leaves, and to detect hairy roots by PCR. Peanut hairy root strains were cultured with five kinds of media(1/2MS, MS, MS +500 mg/L carbenicillin sodium, B5, and N6), the biomass and resveratrol content of hairy root strains were measured by HPLC after four weeks. Results showed that the accumulation of biomass and content of resveratrol of peanut hairy root strains had a significant effect in different kinds media, 1/2MS medium and MS medium were conducive for the biomass accumulation of peanut hairy root strains. B5 medium and N6 medium were conducive for the resveratrol accumulation of peanut hairy root strains.