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作 者 ：张燕 方力 李天飞 姚照兵 冯永新 吴业池

期 刊 ：植物学通报 2002年 19卷 03期 页码：342-347

关键词：钙；烟草幼苗；低温胁迫；保护酶；抗冷性；

摘 要 ：用CaCl2浸种处理烟草种子，研究了钙对烟草幼苗某些酶活性的影响。结果表明：CaCl2浸种能够提高烟草幼苗结合态钙和膜保护酶活性，降低膜透性和MDA（丙二醛）含量。在低温胁迫条件下，Ca2+浸种处理的烟草幼苗SOD、CAT和POD等保护酶活性下降程度较未经处理的轻，细胞相对电导率低。恢复生长后，幼苗膜透性和保护酶活性恢复较快。CaM（钙调素）特异性抑制剂CPZ（氯丙嗪）能部分抑制Ca2+提高SOD、CAT和POD活性的作用。

Abstract:After submerging tobacco seeds with CaCl2 solution, we studied the effect of Ca2+ on activities of certain enzymes in tobacco seedlings. It was shown that submerging tobacco seeds with CaCl2 solution increased the content of combinative calcium in tobacco seedlings and the protective enzyme activity, and reduced the permeability of cell membrane and the content of MDA. Under the low temperature stress, the reduction of SOD, POD and CAT activities in the tobacco seedlings treated with Ca2+ was lower than that of control seedlings . The relative electric conductivity of cells in tobacco seedling treated with Ca2+ also reduced. The recovery of cell membrane permeability of tobacco seedlings treated with Ca2+ was faster than that of control seedlings after seedling growth re sumed. CPZ, the special inhibitor of CaM, could partly restrain the function of Ca2+ in increasing the activities of SOD, CAT and POD.

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