Ethyl methane-sulfonate (EMS)-mutagenized Arabidopsis M2 populations were screened in low-K+ medium using the root-bending assay. Forty-two putative low-K+-tolerant ( lkt ) mutants were selected from 150?000 tested M2 seedlings, and two of these mutants maintained their low-K+-tolerant phenotype in their M3 generations, respectively. Genetic analysis showed that either one of these two mutants has a monogenic recessive mutation in a nuclear gene, and that the two mutations in two independent mutants are allelic to each other.
拟南芥耐低钾突变体的筛选及遗传分析
赵淑清* 林春* 杨小贺 武维华**
（中国农业大学生物学院植物科学系，农业部植物生理生化开放实验室，北京100094）
摘要： 利用乙酰甲基磺酸（EMS）诱变方法，以幼苗根在重力作用下的弯曲生长为指标，筛选得到了拟南芥（Arabidopsis thaliana）耐低钾突变体。经过对突变体杂交后代的遗传分析证明，其中两株突变体的耐低钾性状为隐性单基因突变所致。鉴定、分离与植物耐低钾性状连锁的基因将有可能对培育钾高效作物品种有重要意义。
关键词： 拟南芥；耐低钾；突变体
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