Abstract:Twenty-eight stable RAPD primers were screened out of 140 random primers from the groups of OPE, OPF, OPG, OPU, OPX, OPY and OPZ. Phylogenetic dendrogram was constructed based on 488 RAPD fragments stable amplified in five basic genomes of Aegilops L. and Triticum aestivum L. with the 28 primers. The results showed that genome ABD of wheat and genome S were most consanguineously grouped, genomes C and U had a relatively close relationship, and genome D was distant from all other genomes.