Abstract:mmature haploid embryos (n=21, ABD) were obtained by crossing durum wheat (Triticum durum, 2n=28, AABB) as the female parent and Aegilops tauschii (2n=28, DD) as the male parent. The haploid embryos were rescued by tissue culture. The plantlets regenerated were treated with colchicine to double their chromosomes and the synthetic wheat with winter growth habit were produced. The regeneration percentage of haploid plantlets was 75.81% using 1/2 MS medium only, but 92.44% haploid plantlets were obtained when the immature embryos were cultured on 1/2 MS medium and 1/2 MS medium supplemented with 2 mg/L 2,4-D alternately. The medium alternation was based on the development of immature embryos. If immature embryo developed well, 1/2 MS medium would be used; otherwise 1/2 MS medium containing 2 mg/L 2,4-D would be used. The poorly developed immature embryos grew well on the medium containing 2 mg/L 2,4-D, and then they were moved to 1/2 MS medium to produce more plantlets. double haploid plantlets (AABBDD) were gained by using 0.05% colchicine with method of half-root in plastic shed in winter. To doubling chromosomes in this season has an important reason that temperature is very beneficial for plantlets growing more strongly and having more tillers, all these made it easy to get more seeds of synthetic wheat.