Abstract:A comparative study on the microtubule cold stability of tomato (Lycopersicum esculentum), cucumber (Cucumis sativus), spinach (Spinacia oleracea), beet (Beta saccharifera) and wheat (Triticum aestivum) was carried out by immunofluorescent microscopy. The results indicated that the depolymerization of microtubules in the stomatal guard-cells of chilling sensitive plants, tomato and cucumber occurred under the chilling treatment of 0 ℃–1 ℃ for 3 hrs, but, in mediate cold rtsistant plants, spinach and beer did not so when their cold acclimated seedlings were treated for 3 hrs at 0 ℃ and–5 ℃. The microtubules in the stronger cold resistant winter wheat variety Nongta 139 were maintained in low temperature acclimating period, and could not be destroyed after the cold acclimated seedlings were frozen at –8 ℃for 3 hrs,. The results suggest that the microtubule cold stability is closely related to the cold hardiness of plants.