Abstract:The identification of the progenies of atrazine-resistante transgenic soybeen plants was carried out by using the methods of spraying the leaves directly with atrazine solution, examining the change of leaf fluorescence kinetics curve under a brighter light induction and molecular hybridization. The resistant gene has been found in plants of F2 and F3 generations, it means that the atrazine-resistance psbA gene from black nightshade not only can be transferred to the chloroplast genome of atrazine-susceptible soybean and expressed, but also inherited to the progenies.