Abstract:Immature inflorescence explants of octoploid Triticale cultured on MS medium with 2 mg/l 2,4-D showed high capacity for callus formation (100%) and subsequent plant regeneration on same medium. Chromosomes of regenerated plants were counted. Most of them showed 2n=56 which is a octoploid complement fur octoploid Triticale, but some plants were composed of decaploid cells with 70 chromosomes and aneuploid cells. The chiasma of homologous chromosomes in tip cell was found.