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期 刊 ：中国中药杂志 2003年 28卷 02期 页码：118.

关键词：山茱萸；茎段；植物组织培养；

Keywords:Cornus officinalis, stem, plant tissue culture,

摘 要 ：目的:研究山茱萸茎段组织培养技术,为山茱萸优良株系的快繁建立最佳条件。方法:选择山茱萸优良母株的茎段为外植体,分别在不同的培养基中培养,形成大量丛芽,再诱导单株苗生根成为完整植株。结果与结论:茎段在WPM+6-BA2.0mg·L^-1+ZT0.1mg·L^-1+NAA0.1mg·L^-1培养基上,能形成大量丛芽;在WPM+6-BA1.0mg·L^-1+ZT0.1mg·L^-1+GA30.5mg·L^-1+NAA0.1mg·L^-1中继代培养,可获大量壮苗;切取单株苗在1/2 MS+6-BA0.1mg·L^-1+IBA1.0 mg·L^-1培养基中能生根,移栽并成活。

Abstract:To set up the optimums of tissue culture for Cornus officinalis. Method: A section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone. Result and Conclusion: The WPM medium with 6-BA 2.0 mg·L^-1+ZT0.1 mg·L^-1+NAA 0.1mg·L^-1 was the optimum medium for the multiplication of adventitious buds. The WPM medium with 6-BA 1.0 mg·L^-1+ZT0. 1 mg·L^-1+GA3 0.5 mg·L^-1+NAA 0.1mg·L^-1 was the optimum medium for cultivation in strength. The plantlet could root well in 1/2MS medium with IBA1.0mg·L^-1+6-BA0.1mg·L^-1.

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