Abstract:Six kinds of glycolipid of monogalactosyl and digalactosyl diglyceride were separated and purified from the wheat and the natural latex of chilling-tolerant and chilling-sensitive variety by two kind solvent systems and twice one-dimensional thin-layer chromatography. Besides, the compositions of fatty acid of hydrophobic side chain of six kinds of glycolipid were compared. The indices of unsaturation of fatty acid of hydrophobic side chains in the glycolipids of wheat flour, in the glycolipids of natural latex of chilling-tolerant, variety and in the digalactosyl diglycerides, are much bigger than those, respectively, in the glycolipids of natural latexes, in the glycolipids of chilling-sensitive variety and in the monogalactosyl diglycerides.