Abstract:The content of glucosinolates during anther culture of Brassica junces has been investigated. Experiment results expressed that the content of glucosinolates in pollen embyoids was high, then decreased with the growth and development of the embryoids, A ralative stability of the content was maintained from 30 to 50 days cultured. When the secondary embryoids came into being, the content increased rapidly. The myrosinase activity of every sample was determined at the same time. The results of determination showed that the pattern of the change of myrosinase activity was similar to the glucosinolate content.This indicates that there is a correlation between glycosinolate content and myrosinase activity.