The evolutionary history of the tribe Convallarieae (s．l.) is constructed by comparative RFLP of PCR-amplified chloroplast DNA fragment and subsequent phenetic analyses with UPGMA method．The results show that the genus Tupistra and the genus Aspidistra are phylogenetically closely related，and the genus Theropogon may be alien to the other genera of the tribe，supporting the conclusion derived from morphological，karyotypical and palynologictal aspects．  In addition，the systematic position of the genus Convallaria is also discussed．