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Culture medium optimization for producing cellulase by Trichoderma reesei

里氏木霉Trichoderma reesei产纤维素酶的发酵培养基碳氮源优化



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ChineseJournalofBioprocessEngineering
Vol.9No.4
Jul.2011
doi:10.3969/j.issn.1672-3678.2011.04.002
OPQI
:2011-01-17
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:1672-3678(2011)04-0006-05
CulturemediumoptimizationforproducingcelulasebyTrichodermareesei
ZUCaixia,LIZhimin,YEQin
(StateKeyLaboratoryofBioreactorEngineering,EastChinaUniversityofScienceandTechnology,Shanghai200237,China)
Abstract:Theinfluenceofcarbonandnitrogensourcesoncelulaseproductionwasinvestigated.Single
factormethodandresponsesurfacemethodologywereusedtooptimizethemediumforcelulaseproduc
tionbyTrichodermareesei.Firstly,itshowedthatsoybeanpowder,corncobandcornsteepliquorhad
positiveefectsoncelulaseproductioninonefactoratatime.Then,theoptimalconcentrationsofsoy
beanpowder(3221g/L),corncob(4229g/L)andcornsteepliquor(445g/L)weredetermined
bycentralcompostiondesignandresponsesurfaceanalysis.Undertheseoptimizationsconditions,the
celulaseproductionreached(1065±050)U/mLontheflaskcultivationfor7d.
Keywords:celulase;Trichodermareesei;responsesurfacemethodology
  
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