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Culture medium optimization for producing cellulase by Trichoderma reesei

里氏木霉Trichoderma reesei产纤维素酶的发酵培养基碳氮源优化



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1278C¶p

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10、15
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m1 
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Fig.1 Efectsofsoybeancontentoncelulaseproduction
103U/mL;
Ï/8ÂL
35g/L
û

?@ABËBUO
0kL
33U/mL。
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40g/L
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0
m
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Fig.3 Efectsofcornsteepliquorcontenton
celulaseproduction
kL
37U/mL。
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X1
ρ(½˜¾â)
X2
ρ(ã¬Ç)
X3
ρ(㬿)
-1682 1318 1477 080
-1 2000 2500 250
0 3000 4000 500
1 4000 5500 750
1682 4682 6523 920
 
  
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Table2 Centralcompositedesignandresult
L
X1 X2 X3 Y/(U·mL-1)
1 -1 -1 -1 513
2 1 -1 -1 622
3 -1 1 -1 379
4 1 1 -1 821
5 -1 -1 1 499
6 1 -1 1 290
7 -1 1 1 444
8 1 1 1 654
9 -1682 0 0 512
10 1682 0 0 555
11 0 -1682 0 345
12 0 1682 0 539
13 0 0 -1682 723
14 0 0 1682 572
15 0 0 0 1003
16 0 0 0 993
17 0 0 0 1097
18 0 0 0 1026
19 0 0 0 977
20 0 0 0 1122
  

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Y=103587+04567X1+05124X2-05132X3-
17429X21 -206729X

2 -13383X

3 +09405X1X2 -
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Table3 Varianceanalysisofregressionequation
þÿF C© C©Ìýþÿ
T P
i©Ê
103587 02257 45887 0
X1 04567 01498 3049 0012
X2 05124 01498 3421 0007
X3 -05132 01498 -3427 0006
X21 -17429 01458 -11954 0
X22 -20672 01458 -14178 0
X23 -13383 01458 -9179 0
X1X2 09405 01957 4806 0001
X1X3 -06867 01957 -3509 0006
X2X3 03052 01957 1559 0115
 
  
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m
5 X3=1,X1、X2%FPAcWXY
Fig.5 ResponsesurfaceofX1,X2andFPAwhenX3=1
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páò

8½˜¾â
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ã¬Ç
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û

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Fig.6 ResponsesurfaceofX1,X3andFPAwhenX2=1
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7 X1=1,X2、X3%FPAcWXY
Fig.7 ResponsesurfaceofX2,X3andFPAwhenX1=1
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F*
µù½˜¾â
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ã¬Ç
4229g/L,
㬿
445g/L,
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龿Y³6
C、N
FQ6´š6èÊ

Ý
Yç?@ABR36^À.

M¬6q

[1] 
TU

u°2

xVV

?@AB6*³I¹
[J].
mí±¯

2008(12):1517.
[2] RyuD,MandelsM.Celulases:biosynthesisandapplications
[J].EnzymMicrobialTech,1980(2):91102.
[3] FangXu,YanoS,InoueH,etal.Lactoseenhancescelulasepro
ductionbythefilamentousfungusAcremoniumcelulolyticus[J].J
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‹Ê

ˆWW

uX

Y·=>3?@AB129:6¾¿
[J].
ØÙ^À‰`
,2008(3):128130.
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…Z
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³çm1Òµyz·©‹œ
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ÿˆ[·¾¿
,2007,7(3):231234.
[15] 
‹]¶

‹^
.RPHPLC
²¼½˜¾âm6

_@RA
[J].
.ˆ·RS^Ä
,2008,25(5):7375.
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[17] 
`ab

x“^

㬿e°>A12R36})
[J].

ƒÿ^Àž
,2006,37(8):528530.
[18] 
‹šO

’í“

cd

e<=>
ZJ5
?@AB1278C
6-.
[J].
fg~ˆˆt
:^
ˆ„
,2003,37(5):623627.
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