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Separation and purification of oleuropein from olive leaves

油橄榄叶中橄榄苦苷的分离纯化



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ChineseJournalofBioprocessEngineering
Vol.9No.4
Jul.2011
doi:10.3969/j.issn.1672-3678.2011.04.007
OPQI
:2010-12-09
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Separationandpurificationofoleuropeinfromoliveleaves
FUShan,WANWenqian,YANGWenge,HUYonghong
(ColegeofBiotechnologyandPharmaceuticalEngineering,NanjingUniversityofTechnology,Nanjing210009,China)
Abstract:Extraction,purificationandseparationofoleuropeininoliveleafextractwerestudiedby6dif
ferentmacropornsresins.Macroporousabsorptionresinswerecomparedonadsorptionanddesorptionper
formances.Toachieveanoptimalpurificationofoleuropein,theadsorptionanddesorptioncharacteristics
ofthebestresinwereinvestigated.TheoptimalcolumnseparationconditionsofmacroporousresinD101
wereasfolows:themassconcentrationofoleuropeinintheliquidwas4106mg/mL,theflowratewas
5mL/min,50% aqueousethanolsolutionwasefectivesolventforoleuropeindesorption,thevolumeof
eluentwas260mL.Thecontentofoleuropeinwasmorethan55%,theoleuropeinwasobtainedbypuri
ficationagainusingSephadex,and76% yieldwasobtained.
Keywords:columnchromatography;macroporousresin;oleuropein
  
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lÛm

G

žûnxijklmjkno
\]pqf‹Œo¼
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Îp

:Û7

†qr

G

jkno\]pqÿUN6
¾¿º»
[J].
sփˆ@ˆt
,2010,27(1):6972.
[5] 
xt[

‹:«

ÛÜ3SmUN¶p\]`a¾¿º»
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,2003,5(4):2933.
[6] 
<·ú

]ŠÐ

Hu

G

úû³~€‚ƒh.w4
Ý6›,
[J].
Øى`
,2010,35(8):251254.
[7] 
x[³

v7

cwx

G
.D101
E~€‚ƒh.iwy
o6^_¾¿
[J].
míÿ›
,2009,20(12):900901.
[8] 
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!Þ_`·’,
(HPLC)
}ÛÜ3S
m6*³
[J].
~|.^
,2009(1):2731.
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