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Recent progress of biorefinery of polyols based on biomass resources

基于生物质资源的化工多元醇生物炼制研究进展



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2013
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ChineseJournalofBioprocessEngineering
Vol.11No.2
Mar.2013
doi:10.3969/j.issn.1672-3678.2013.02.007
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:2012-12-29
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CHIB
:1672-3678(2013)02-0044-08
Recentprogressofbiorefineryofpolyolsbasedonbiomassresources
MACuiqing,LILixiang,GAOChao
(StateKeyLaboratoryofMicrobialTechnology,ShandongUniversity,Jinan250100,China)
Abstract:Themainuseandmarketdemandsof2,3butanediol,1,2propanediolandxylitolwere
presented.Therecentdevelopmentinbiorefineryproductionof2,3butanediol,1,2propanedioland
xylitolwassummarized,includingtheproducingstrains,rawmaterials,andproducingmethod.
Keywords:polyols;2,3butanediol;1,2propanediol;xylitol;biorefinery
  
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a

ÚD(—’&q
2,

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ªn
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[27]
ij¹

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coli)
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。Yan
a
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a
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~
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。Li
a
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a
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059g/(L·h)。
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wSD8Äࣃ&qZwˆ)BNçè

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tQ4/NZw~:;waw®
,Wang
a
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wS™éâ
,^

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L÷ôM

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2,3
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†N(>Å­E\¹

m
4%NaOH
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œlTâh
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a
[43]
ÐbP8Ä
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‡?_£]¹NbcdLՋŒãN
Cu ZnO
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•^ôM×҇‘½*\:¾
992%,
1,2
¤ŸˆjN“¾
925%。
Luo
a
[45]
:;Ž
Ru B
^™ÝrN γ Al2O3
TâE\҇‘½q
1,2
¤ŸˆN“U

æ‚h
Ôþst€ðNE\(>“U

Ñ(C|¹ìN
Sn
Ih23k(҇‘½N*\:~jN“

G-¾
¹
907%
~
915%,
Õ¸
Sn
N(IhçQTâ
NGõ“~w—“

ðSɲ҇&qª6(
Q

ßUVGH“LQ

3.4 
Ï+,2Á+b
1,2
TQI
ɲ

qáNIq
1,2
¤ŸˆNF&XY÷
~‘’
[46]。SanchezRiera
a
[47]
:;ŽX/
、pH、
ù4/aAÓR΢‡’
(Clostridium
thermobutyricum)
L÷q
1,2
¤ŸˆN"#

h:;
w™ù

m•^ôM×þqìI¾
9g/L。Suzuki
a
[48]
L¡÷†stq
1,2
¤ŸˆNU†

Altaras
a
[49]
m
E.coli
{àjŽ(S:;wN
1,2
¤ŸˆCªª«

ÙÞºŽáے҇Cª
%(A

‡âG$L÷{
1,2
¤Ÿˆqì¾¹
45
g/L,
R:;wN*\:™
019g/g。
Clomburg
a
[15]
àjŽI8Ä1à&q
1,2
¤
ŸˆN
E.coli
)+’

þqìI¾
5.6g/L。Nimi
a
[13]
m/:‡Ž’
(Corynebacteriumglutamicum)
{h¾„V
E.coli
N
mgs,
þ
1,2
¤ŸˆqìçQ
Ž
100
3

¾¹
93mmol/L,
ÐbP‰h¾H$´
éJ
(aldoketoreductase),
¼
1,2
¤Ÿˆqì¾¹
24mmol/L。
ɲ
,1,2
¤ŸˆNL÷_qì~*\:aê
.(n

*«‰)B\±‚´t(vN*^

Õ
¸yÃ&:;(™ñ

4 
‚ƒI+,~•žŸø3
ɲ

¬­öۂZwˆ&q8B

"É
è„~h~$ÐúBtž}~

@Ä\}(>_
Ð{Zwˆ&q

þéâG-™‘Z~ࣃ

74 
!

# JRSa

(S&-ëVN\)y”ˆ&У:;ÐM
þ{R[\ÓG‡ê­
、^
H¤×



æEa
‘\À¹Zw

À(>¹Zwˆ
[22]。
Ú
D\}_Ïmðñv

UîQaX°

p›&ã

&_ۂD8ÄࣃR[\Óê­SG*
=þzûLG*=þ†°ÄÞL÷ù

Ð{Z
wˆN&q

l<_ðñ÷

Uîn

DZwˆq
BLMN<=

4.1 
+bÍl
u­{ÏmbüUeL÷ZwNF&


ÚvœG˜þ*\™&ìi‘ˆa¨q

Zwˆ†^™L÷N{4q›b

‘’

÷~
=’{ƒL¡t÷œG’IUeî$Zwˆ

4.1.1 
ÄSZwˆ&qNÍ&’Æ
ɲ

qáNqZwˆNF&XY‘’{N
_’GՒ
(Myobactenumsmegmatis)、
Ž~’
W
(Corynebacterium),
¿ ~ ƒ ’ { N < =
(Penicilium)、
â=
(Aspergilus)、
Z=
(Trichoderma)
~÷

þ{÷*\Zw&qZwˆN“U(
™^;

¡:;(yNDKß8÷W{N“”
Kß8÷
(Debaryomyceshanseni)、
¾¿÷W{
N•†–¾¿÷
(Candidaguiliermondi)、
Á.—
¾¿÷
(C.parapsilosis)、
ü¾¿÷
(C.
tropicalis)
~´˜¢¾¿÷
(C.boidini)
a
[22]。
{‚Ôa
[50]
+Š‹{ij¹ISæQ4/
ZwN
C.tropicalisSB18
’Æ

mÐò,I{

ð
250g/L
ZwL÷
252h
¹
2076g/L
Zwˆ

*
\:™
831%,
¾¹AfòN
918%。
Cheng
a
[51]
ij¹

ÆqZwˆN÷
C.tropicalisW130,
Ù^\Žl’ÆL÷ZwˆNô
M

m‡âG$‘|{8ĵaYण•‰¹
Zwˆqì™
218g/L。
Ling
a
[52]
8ÄÇÈ}<_^\Ž
C.tropicalis
HDY 02
8ÄࣃR[\Óê­SL÷qZw
ˆN‘|(

Ùm•^‘|({Ð{ŽqK‡âL
÷

ZwˆqìI¾
58g/L,
ZwˆRZwN*\:
™
07g/g。
4.1.2 
ÄSZwˆ&qN(A)+’Æ
mijÍ&Zwˆ&q’ÆNÕ¸

™ŽçQ
’ÆNqì

*\:a

:;4î´¨MŽ&qZ
wˆ(A)+’ÆN:;

͙ša
[53]
8Ä¡•÷
(Saccharomyces
cerevisiae)
hpMZcÇ

˜„VS
C.tropicalis
N
xyl1
øüt
His Tag
N¡•÷α š¬ÓMZ
Ýr
pICAS his
{

uB¡•÷U*\Zw&q
Zwˆ

*\:¾
98.7%。
ß7a
[54]
8ÄQ›œ-O˜: ßà÷
(Pichiastipitis)
Zw´éJ(A
xyl1
*\¹’Æ
S.cerevisia6508
{

¹›Q›œZw´éJ(A
NuB’Æ
XGH2,
ÐòL÷*+{íîZw
279
g/L,
q&Zwˆ
302g/L。
Guo
a
[55]
˜Šâ={NZ†w(A
atn
~Zw
‹J(A
atl
m
C.tropicalis
{ªF‰h¾

l’Æ
I†°8ÄZ†wzࣃ^™éâL÷qZw
ˆ

þ*\:G-I¾¹
771%
~
669%。
ɲ

qZwˆN)+’Æۂh‘Zwzû
Z†wa™éâ

mR†°8ÄࣃR[\Óê
­SN)+’Æqá(÷

4.2 
Ž¥ì/‘’è
ÉyD¬­žvà£&qy›b

Ê$Ihq
&
2000
Ø

Nࣃ

8Äࣃ^™éâÐ{
ZwˆN&q©ty$

8Bxy

þ{h~$Ð
úBtž}~™˜$•vNZwˆ&q8B

s]
35
Ø

ZwˆN&qU†

ࣃNÜ A)WۂXYR[\Óéâ
Nê­~ê­S=þ

4.2.1 
ࣃR[\ÓNê­
‡ê­Dɲ•12ËNࣃR[\Ó
ê­<_
。Wang
a
[56]
:;Ž”
H2SO4Rࣃê
­ª2wN"#

æ‚hÔm}Sã™
05
NôM
×
,160~170℃
™(|CNX/

m
170℃、2.2%
N
H2SO4 A30min,Zw«:™à£ƒN236%,
¾¹Af«:N
75%。
Wang
a
[57]
8Äo‡Ý¢
H2SO4Ð{Žà£ƒ
R[\Óê­N:;

æ‚2ZmFÀ’å×Är
îG}
60%
No‡m
100℃
ôM× A
2h,
Zw
N«:I¾
963%,
ùLÄFÀ’å AôM×Z
w«:U¾¹
751%。
Õ¸FÀ’åôM×Z
-Óáº:U¾¹
717%,
!QSòôM×N
319%。
læ‚hÔFÀæCo‡ A†DbÚt
ÎNࣃR[\Óê­
‡_RR[\ÓNê­ótÎ

چF
¤ªê­S{^H¯/NXQ
、pH
Nïnhi»£
Nk(

+ùk(ŽÀ ANÉ/
。Wang
a
[58]
˜G*rîG}
01% H2SO4Ü ANࣃmQ
X× Ab—¸4

¹Ž(Q4/NR[\Óê
­S

ÙR AX/
、¸
4~}SãäÐ{Ž^,
L¡m
160℃、
}Sã
1∶20
NôM× A
2h,´
é
w~Zw«:G-¾¹à£ƒN
243%
~
201%。
QXC_p”
H2SO4_&ã,µûq&Ndw&
Ê

G-ÙࣃN
306%
~
314%,
ùQXC
84
&
 

 
(
 
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_¹NR[\Óê­S{®»£›ì~^H
¯/23nS
H2SO4_¹N,œ pH(Q,t8S
L÷8Ä



QXCDbIYZïðNà£
ƒR[\Óê­<_

4.2.2 
ࣃR[\Óê­SN=þ
ࣃR[\ê­S{((›tR’Æ&|
L8Nȣ

XY‘‡

žHa-

RSà£
ƒR[\Óê­S=þN:;ۂXYA\}
_~&_

þ{A\}_D8ÄCŸzû^H
¤×a5aïn»£4/

ù&_ۂDè*
çQ’ÆStþAHU†„,¡N

Ding
a
[59]

D301
:zRࣃê­S{
»£Ð{=þ

 AÀ‘‡

žH~

ao(ž
HG-ïn
757%、553%
~
50%,
ù2wØú
Ž
32%;
ÐbP:;Žè¢Ë/R
Candidasp.
ZU04
ZwˆL÷N"#

L¡L÷²#QYàt8
S’r&|

ùÀ#nYàtåSZwˆNî$

m^\NµÐaYणºP{Zwˆ•Qqì
¾¹
60g/L,
ZwˆRZwN*\:™
076g/g。
Cheng
a
[60]
æCQXCL
、Ca(OH)2 Ai‘
‡‘½ïÅRࣃR[\Óê­SéâÐ{Ž
=þ A

 AÀžH~

ao(žH(6€L
¹

ÙÐbP8Äléâ:;Ž‘‡~
pH
R
C.
tropicalis
L÷ࣃR[\Óê­SqZwˆN"
#

m•^ôM×


72h
Zwˆ¾¹•vqì
684g/L,
ZwˆRZwN*\:™
07g/g。
Wang
a
[58]
8ÄࣃQXCSRZwˆL
÷’Æ
C.tropicalis
Ð{ŽÛ,
çQŽ’ÆSæt
þAHNU†

þZwˆ*\:(éQ’ÆçQ
25
3

¾¹
07g/g。
8ĵÐaYणºP‡
âG$L÷

ZwˆqìI¾
98g/L,
*\:I¾
714%,
&q¯/¾¹
21g/(L·h),
ãࣃ‡ê
­SNq:~&q¯/G-çQŽ
158%
~
149%。
G¶2ZQXCS{N»£AH4/(‡ê­
Sn

†hÔQXCN<_DbÚYZïð~Q
ÎNࣃ A<_

ðSA\}_ A*+2S

G¤ªðñ

Uî(Q

ù&_è*çQ’ÆSæŽI,¡R
ࣃR[\Óê­SNTÄ

Aß8ÄGH&
}

¢“)+a5açQ’ÆRtþAHSæ“D
ÁÀNbÚ:;<=

4.3 
2Á~
ɲ

ZwˆL÷&q
‡âG$L÷~}—\‘—L÷a

©t:;hÔ
R[\Óê­S{((›tbüR÷’L÷L
8NªG

"‘‡

žHa

AßG$L÷~‡âG
$L÷
}—\‘—st‘—¬/Q

KT

-ðñU†
¯

IqK¼Ä

qG^<Ûa,°

mR[\Ó
ê­SéâL÷{st^

Œ a
[61]
8ÄÌ¥‡jš›}—
C.tropicalis
‘—L÷ࣃR[\Óê­S

Ù^\ŽL÷)
W

8Ä}—\‘—u2Ð{
10
$Ɖ
30d


Zwˆ:.ƒ™
73.7%,
¾¹ŽAfòN
80%。
}—\‘—I8ăG=mp^H¤×Nê­S
ÛI*\ªZwˆ

2ZŽ€ðNTIJ%

Wang
a
[62]
8ÄUt^™}—\Tâ
,^

´Q‘—4/
、pH、
X/a·}

8ÄࣃR[Óê­S&qZwˆ

m
21d
NL÷{

Zwˆ•
v*\:~&q¯/I¾
712%
~
21g/(L·h),
10
$Æ.ƒ*\:¾¹
66.3%,
&q¯/¾¹
19
g/(L·h),
þqì°Á
100g/L。
læ‚hÔUt}
—\‘—_DbÚtÎNZwˆ&q<_

5 
“˜™«3¬
Á$„

\)y”ˆTÄ?CNLMLM

,
-D(S\)y”ˆ†CTâ&q)WN¨L:
;

¼\)y”ˆN±²½Œkv

&У?
CcCIJKLMOP±²

¹>:)^ûNÖ
{5

Éym
2,3
¢Ÿˆ
、1,2
¤Ÿˆ~Zw
ˆa\)y”ˆ&У×ØŎ(vNÐM

œG:;¾¹Žyõ¿Ðê.

ÚDɲÉy\)
y”ˆ×Ø´Ïmbü®¯

RS
2,3
¢Ÿˆ„‚

ï8Ä:;w^™

Vmqì

&q¯/~*\:N+/

Úþ&qéâNÒÌ(™!"

67e
\&qNGH“LQ

ù8ĝÒéâ

"Z-[
\Ó®éâ

Nqì

&q¯/~*\:(n

GH
“ÕpLQ

Õ¸


2,3
¢ŸˆN&qê.Ã
Ä(n
,´
±‚ÐbP:;5“
2,3
¢ŸˆNѪ
Þ£

Ù8Ä¢“)+a5açQþqì

&q¯
/a

mqB\:;
ðS
2,3
¢ŸˆN&q
æYàê."#óv

"+mveL÷rc{,
¡YàN!Õ¤£DbÚɯ

RS
1,2
¤Ÿˆ„‚

ɲۂ:;ND\}
_E\&-éâ":;wzû1àÐ{
1,2
¤Ÿ
ˆN&q

œ©G,¡ŽqB,
ÚD´Ïm\}E
\¹w—“LQ

±‚—¸5×a®¯

yõöR
1,2
¤ŸˆNL÷’ÆÐ{Žy$N:;

è*’
Æij

¢“)+K¤a5a,¡Ž
1,2
¤ŸˆN
94 
!

# JRSa

(S&-ëVN\)y”ˆ&У:;ÐM
L÷_&q

ÚþL÷ê.ón

Éh«‰ve&
qN±‚

Aß´±‚ÐbP(¯ß
RSZwˆ„‚

ɲy÷m’Æij~K
¤
8đZw^™


ZwˆqìÛ*
200g/L,
ÚDðSR[\Óê­S
éâ{((Ïmbü»£AH

"‘‡

žHa

8
ÄR[\Óê­SéâL÷ZwˆNqì

&q¯
/~*\:(n

Aß±‚ÐbPRR[\Óéâ
NÜ A
ïn»£AH4/

çQ
*\:

8ÄM+’I

¢“)+K¤a5açQ
’ÆS&tþAHNU†

+ùçQqì

&q
¯/~*\:

,¡R[\ÓéâNQÎ8Ä

y”ˆNG^†Dɲ\)y”ˆ&q{N
É°
,¨
LQÎ

GHNçÅ5aRSveN&
qstu‚wx

ÚDɲ&:;(÷

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ÁtIU,¡
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¼y”ˆƒlª™.HC

­®CD

[1] JiX J,HuangH,OuyangP K.Microbial2,3butanediol
production:astateoftheartreview[J].BiotechnolAdv,2011,
29(3),351364.
[2] ZengAP,SabraW.Microbialproductionofdiolsasplatform
chemicals:recentprogresses[J].CurOpinBiotechnol,2011,22
(6):19.
[3] 
{yêµÓ¢:;{O
.2010~2011
${yŸ”ˆêµLM
:;qr
[EB/OL].[20121223].htp:∥www.cmir.com.
cn/Html/hot_report/2012072324238471.shtm
[4] 
æç

h¿

&УLM¡~i²%M&
[J].
¡¢\)

2006,26(4):611.
[5] XiuZL,ZengAP.Presentstateandperspectiveofdownstream
processingofbiologicalyproduced1,3propanedioland2,3
butanediol[J].ApplMicrobiolBiotechnol,2008,78(6):
917926.
[6] GargSK,JainA.Fermentativeproductionof2,3butanediol:a
review[J].BioresourTechnol,1995,51(2/3):103109.
[7] CelińskaE,GrajekW.Biotechnologicalproductionof2,3
butanediol:curentstateandprospects[J].BiotechnolAdv,
2009,27(6):715725.
[8] XiaoZJ,XuP.Acetoinmetabolisminbacteria[J].CritRev
Microbiol,2007,33:127140.
[9] 
棤

¥jy

Q%

a

F&L÷_&q
2,3
¢ŸˆN
:;ÐM
[J].
¡¢\)
,2006,26(8):2327.
[10] SyuM J.Biologicalproductionof2,3butanediol[J].Appl
MicrobiolBiotechnol,2001,55:1018.
[11] KpkeM,MihalceaC,LiewF,etal.2,3butanediolproduction
byacetogenicbacteria,analternativeroutetochemicalsynthesis,
usingindustrialwastegas[J].ApplEnvironMicrobiol,2011,77
(15):54675475.
[12] 
+¹<

%k¯

hj.

&Ãà&qiþ¨q1àNt
Î8Ä
[J].
{yàz
,2007,32(5):4751.
[13] NimiS,SuzukiN,InuiM,etal.Metabolicengineeringof1,2
propanediolpathwaysinCorynebacteriumglutamicum[J].Appl
MicrobiolBiotechnol,2011,90(5):17211729.
[14] 
ټ
.1,2
¤Ÿˆy÷&q¡~iLM²%
[J].
}“
:z
,2009,24(1):5862.
[15] ClomburgJM,GonzalezR.MetabolicengineeringofEscherichia
colifortheproductionof1,2propanediolfromglycerol[J].
BiotechnolBioeng,2011,108(4):867879.
[16] 
¦j

ªç

§g

a
.Ru/TiO2E\¹ö1à>­£1,2 ¤
Ÿˆ
[J].
E\}q
,2011,32(9):15451549.
[17] EmodiA.Xylitol,itspropertiesandfoodapplication[J].Food
Technol,1978,32:2032.
[18] AkinterinwaO,KhankalR,CirinoPC.Metabolicengineeringfor
bioproductionofsugaralcohols[J].CurOpinBiotechnol,2008,
19(5):461467.
[19] 
¦k¯

h‚ü

ZwˆN,“im]^{NTÄ
[J].
]^
>}
,2004,25(11):379381.
[20] 
÷¨ƒ

ZwˆN&q”?CiþTÄ
[J].
\)>?êµ

2005(2):16.
[21] BruggemanJP,BetingerCJ,NijstCLE,etal.Biodegradable
xylitolbasedpolymers[J].AdvMater,2008,20:19221927.
[22] SilvioS S,AnujK C.Dxylitol:fermentativeproduction,
application and commercialization[M].Berlin:Springer
verlag,2012.
[23] WerpyT,PetersonG,AdenA,etal.Topvalueaddedchemicals
from biomass:volumeI.resultsofscreeningforpotential
candidatesfromsugarsandsynthesisgas[EB/OL].[201212
20].htp:∥ www.fischertropsch.org/DOE/DOE_reports/
35523/Vol.1/Werpy%20%2035523.pdf.
[24] FondO,JansenNB,TsaoGT.Amodelofaceticacidand2,3
butanediolinhibitionofthegrowthandmetabolismofKlebsiela
oxytoca[J].BiotechnolLet,1985,7:727732.
[25] MaC,WangA,QinJ,etal.Enhanced2,3butanediolproduction
byKlebsielapneumoniaeSDM[J].ApplMicrobiolBiotechnol,
2009,82(1):4957.
[26] JiXJ,HuangH,DuJ,etal.Enhanced2,3butanediolproduction
byKlebsielaoxytocausingatwostageagitationspeedcontrol
strategy[J].BioresourTechnol2009,100(13):34103414.
[27] YangT,RaoZ,ZhangX,etal.Productionof2,3butanediolfrom
glucosebyGRASmicroorganismBacilusamyloliquefaciens[J].J
BasicMicrobiol,2011,51(6):650658.
[28] GaoJ,XuH,LiQJ,etal.Optimizationofmediumforonestep
fermentationofinulinextractfrom Jerusalem artichoketubers
using Paenibaciluspolymyxa ZJ9 to produce R,R2,3
butanediol[J]BioresourTechnol,2010,101(18):70877093.
[29] WangQ,ChenT,ZhaoX,etal.Metabolicengineeringof
thermophilicBaciluslicheniformisforchiralpureD2,3
butanediolproduction[J].BiotechnolBioeng,2012,109(7):
16101621.
[30] JiXJ,HuangH,ZhuJG,etal.EngineeringKlebsielaoxytocafor
eficient 2, 3butanediol production through insertional
inactivationofacetaldehydedehydrogenasegene[J].Appl
05
&
 

 
(
 
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11
"
 
MicrobiolBiotechnol,2010,85(6):17511758.
[31] ZhangL,SunJ,HaoY,etal.Microbialproductionof2,3
butanediolbyasurfactant(serawetin)deficientmutantof
SeratiamarcescensH30[J].JIndMicrobiolBiotechnol,2010,37
(8):857862.
[32] YanY,Lee,CC,LiaoJC,.Enantioselectivesynthesisofpure
(R,R)2,3butanediolinEscherichiacoliwithstereospecific
secondaryalcoholdehydrogenases[J].OrgBiomolChem,2009,7
(19):39143917.
[33] LiZJ,JianJ,WeiXX,etal.Microbialproductionofmeso2,3
butanediolbymetabolicalyengineeredEscherichiacoliunderlow
oxygencondition[J].ApplMicrobiolBiotechnol,2010,87(6):
20012009.
[34] SunLH,WangXD,DaiJY,etal.Microbialproductionof2,3
butanediolfrom Jerusalem artichoke tubers by Klebsiela
pneumoniae[J].ApplMicrobiolBiotechnol,2009,82(5):
847852.
[35] LiD,DaiJY,XiuZL.Anovelstrategyforintegratedutilization
ofJerusalem artichoke stalk and tuberforproduction of
2,3butanediolbyKlebsielapneumoniae[J].BioresourTechnol,
2010,101(21):83428347.
[36] WangAL,XuYQ,MaCQ,etal.Eficient2,3butanediol
productionfrom cassavapowderbyacropbiomassutilizer,
Enterobactercloacaesubsp.disolvensSDM[J].PLoSOne,2012,
7(7):e40442.doi:10.1371/journal.pone.0040442.
[37] ChengKK,LiuQ,ZhangJA,etal.Improved2,3butanediol
production from corncob acid hydrolysate by fedbatch
fermentationusingKlebsielaoxytoca[J].ProcessBiochem,2010,
45(4):613616.
[38] WangA,WangY,JiangT,etal.Productionof2,3butanediol
fromcorncobmolasses,awastebyproductinxylitolproduction
[J].ApplMicrobiolBiotechnol,2010,87(3):965970.
[39] JiXJ,NieZK,HuangH,etal.Eliminationofcarboncatabolite
repressioninKlebsielaoxytocaforeficient2,3butanediol
productionfrom glucosexylosemixtures[J].ApplMicrobiol
Biotechnol,2011,89(4):11191125.
[40] 
©|Ü

ߪ€

!þh

a

h…ˆ£]i*\E\¹:;
ÐM
[J].
\)&qp?C
,2007,14(1):3437.
[41] 
åo1

bIðh…ˆÅ­&qŸ”ˆ~y”ˆN<_

{
y
,1683293[P].20051019.
[42] HuangZ,CuiF,KangH,etal.Highlydispersedsilicasupported
coppernanoparticlespreparedbyprecipitationgelmethod:a
simplebuteficientandstablecatalystforglycerolhydrogenolysis
[J].ChemMater,2008,20(15):50905099.
[43] FangC,ChenJ,XiaC,etal.Methodforproducing1,2propylene
glycolusingbiobasedglycerol:US,20090156866[P].2009
0618.
[44] 
‰Š

«$Þ

o–.
.Cu ZnO
E\¹ÄS҇‘½€
&(>£
1,2
¤ŸˆN:;
[J].
¡¢\)
,2011,31(5):
7982.
[45] LuoG,YanS,QiaoM,etal.EfectoftinonRuB/γAl2O3
catalystforthehydrogenationofethyllactateto1,2propanediol
[J].ApplCatalA,2004,275:95102.
[46] SaxenaRK,AnandP,SaranS,etal.Microbialproductionand
applicationsof1,2propanediol[J].IndianJMicrobiol,2010,50
(1):211.
[47] SanchezRieraF,CameronD C,CooneyC L.Influenceof
environmentalfactorsintheproductionofR()1,2propanediol
byClostridiumthermosaccharolyticum[J].BiotechnolLet,1987,
9:449454.
[48] SuzukiT,OnishiH.AerobicdissimilationofLrhamnoseandthe
productionofLrhamnonicacidandpropanediolbyyeast[J].
AgricBiolChem,1968(32):888893.
[49] AltarasNE,CameronDC.Enhancedproductionof(R)1,2
propanediolbymetabolicalyengineeredEscherichiacoli[J].
BiotechnolProg,2000,16:940946.
[50] 
{‚Ô

¬_8

­Æx

a

QqZwˆ÷’Nijñ—
iµY_L÷,“
[J].
®|v}}q

u>}¨
,2011,50
(3):624630.
[51] ChengKK,LingHZ,ZhangJA,etal.Strainisolationandstudy
onprocessparametersforxylosetoxylitolbioconversion[J].
BiotechnolBiotechnolEquip,2010,24:16061611.
[52] LingH,ChengK,GeJ,etal.Statisticaloptimizationofxylitol
productionfromcorncobhemicelulosehydrolysatebyCandida
tropicalisHDY02[J].NewBiotechnol,2011,28(6):673678.
[53] 
͙š

LQe

Þ"

a
.,
ü¾¿÷Zw´éJm¡•
÷‘—hpMZ
[J].
]^pL÷)B
,2008,34(5):
2934.
[54] 
ß7



&þl

a

w—QÎh¾Zw´éJ(A)B
¡•÷NàjiZwˆL÷wP:;
[J].
]^pL÷)
B
,2006,32(1):15.
[55] GuoX,ZhangR,LiZ,etal.Anovelpathwayconstructionin
Candidatropicalisfordirectxylitolconversionfromcorncobxylan
[J].BioresourTechnol,2012,128C:547552.
[56] WangGS,LeeJW,ZhuJY,etal.Diluteacidpretreatmentof
corncob foreficientsugarproduction[J].ApplBiochem
Biotechnol,2011,163(5):658668.
[57] WangLL,JiangYJ,LiCH,etal.Microwaveassistedhydrolysis
ofcorncobforxyloseproductioninformicacid[C].InMaterials
forRenewableEnergy&Environment,2011,2:332335.
[58] WangL,MingY,FanXG,etal.Anenvironmentalyfriendlyand
eficientmethodforxylitolbioconversionwithhightemperature
steamingcorncobhydrolysatebyadaptedCandidatropicalis[J].
ProcessBiochem,2011,46(8):16191626.
[59] DingX,XiaL.Efectofaerationrateonproductionofxylitolfrom
corncobhemicelulosehydrolysate[J].ApplBiochemBiotechnol,
2006,133(3):263270.
[60] ChengKK,ZhangJA,LingHZ,etal.OptimizationofpHand
aceticacidconcentrationforbioconversionofhemicelulosefrom
corncobstoxylitolbyCandidatropicalis[J].BiochemEngJ,
2009,43,2:203207.
[61] 
Œ 

<°$

±¯Ô

}—\‘—L÷R[\Óê­SqZ
wˆN:;
[J].
Þq\}p)B
,2004,24(1):2933.
[62] WangL,WuD,TangP,etal.Xylitolproductionfromcorncob
hydrolysateusingpolyurethanefoamwithimmobilizedCandida
tropicalis[J].CarbohydrPolym,2012,90(2):11061113.
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