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Isolation, characterization, and stabilization of photosystem I from Spirulina platensis

钝顶螺旋藻(Spirulina platensis



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ChineseJournalofBioprocessEngineering
Vol.8No.1
Jan.2010
doi:10.3969/j.issn.1672-3678.2010.01.012
OPQI
:2009-04-20
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:1672-3678(2010)01-0061-05
Isolation,characterization,andstabilizationofphotosystemI
fromSpirulinaplatensis
YANGFeng,GEBaosheng,LIUShuang,YUDaoyong
(CenterforBioengineeringandBiotechnology,ChinaUniversityofPetroleum,Qingdao266555,China)
Abstract:PhotosystemI(PSI)ofSpirulinaplatensiswasisolatedandpurifiedwithsucrosedensitygradient
centrifugationusingTritonX100asasolubilizingagent.Thesolubilizingratioofdetergentconcentrationto
chlorophylaofthylakoidmembranewasoptimizedandfourpigmentbandswereobtained.Eachbandwas
analyzedbytheabsorptionspectrum,thefluorescencespectrum,andSDSPAGE.TheintactfunctionalPSI
trimerwasidentifiedasthebotombandwithoxygenconsumptionreached420μmol/(mg·h).Circular
dichroismspectrumwasusedtocharacterizethestabilityofPSIwiththeheattreatment.
Keywords:photosystemI;Spirulinaplatensis;stability
  
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