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Preparation of oleanolic acid from Swertia mileensis and its purity determination

青叶胆中齐墩果酸的制备工艺及其纯度测定



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ChineseJournalofBioprocessEngineering
Vol.10No.4
Jul.2012
doi:10.3969/j.issn.1672-3678.2012.04.009
OPQI
:2011-05-03
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:1672-3678(2012)04-0038-04
PreparationofoleanolicacidfromSwertiamileensisanditspuritydetermination
LIKaiquan1,2,ZENGXiaomin3
(1.KeyLaboratoryofProvinceforResearchonActiveIngredientsinNaturalMedicines,Yichun336000,China;
2.ChemicalandBiologicalEngineeringDepartment,YichunUniversity,Yichun336000,China;
3.EducationScienceandTechnologyPark,YichunUniversity,Yichun336000,China)
Abstract:TheextractionprocessofoleanolicacidfromSwertiamileensiswasinvestigatedanditsstructure
wasidentified.SamplesofSwertiaemileensisHerbawererefluxedwith95% ethanolfor2times,eachtime
for15h.AftertreatedwithYCXY2andactivatedcarbonfor30min2times,thecondensatewassepara
tedandtherefinedoleanolicacidwasobtained.Theoleanolicacidwasconfirmedbyphysicochemicalcon
stantdetermination,infraredspectrumanalysis,massspectrometricanalysis,andNMRspectrumanalysis.
Keywords:extraction;oleanolicacid;Swertiamileensis
  
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