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作 者 ：辜夕容,黄建国*

期 刊 ：生态学报 2010年 30卷 2期 页码：357~363

关键词：外生菌根真菌；铝；磷；钾；草酸；

Keywords:ectomycorrhizal fungus（ECMF）, aluminum, phosphorus, potassium, oxalate,

摘 要 ：试验研究了在铝胁迫条件下，6种（株）外生菌根真菌(ECMF)的生长、草酸分泌，以及磷、钾、铝的吸收状况。结果表明，铝对抗（耐）型菌种Pt 715、Hr Sp、Cg SIV的生长无抑制作用，但显著抑制敏感型菌株Lb S238N、Lb S238A和Lb 270的生长，说明ECMF对铝胁迫的生长反应可能是筛选抗（耐）铝的指标之一。在铝胁迫条件下，无论是抗（耐）型还是敏感型菌种（株），都会发生一系列有益于抗（耐）铝的生化反应，包括草酸分泌量、菌丝磷和钾含量增加，H+分泌改变等。在培养液中，草酸电离产生的H+仅占H+总浓度的少数，说明溶液中H+的主要来源不是ECMF所分泌的草酸，而是菌丝细胞为保持吸收阳离子的电荷平衡排出的H+或分泌的其它有机酸。

Abstract:Six ectomycorrhizal fungi (ECMF) were grown in liquid Pachlewski culture medium added with and without Al to study their growth, oxalate exudation, and uptake of aluminum, phosphorus and potassium. Al stress showed no growth inhibition for Al-resistant fungi, Pisolithus tinctorius 715, Cenococcum geophilum SIV and Hebeloma repandum Sp, in contrast to Al-sensitive ones, Laccaria bicolor S238A, L. bicolor S238N and L. bicolor 270, which behaved otherwise in same case. ECMF used in the present experiment were positively responsible to Al stress via changes in proton efflux and increase in oxalate exudation and concentrations of phosphorus, potassium and aluminum in hyphae, whereby could improve the ability of Al resistance. Protons ionized from oxalate accounted for only small amount of protons released by fungi into liquid culture medium, suggesting the proton efflux due to cation absorption by hyphae for charge balance and the ionization of some other organic acids produced by ECMF.

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