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作 者 ：卢萍*,,Dennis Child,赵萌莉,Dale R Gardener,吕桂芬,韩国栋

期 刊 ：生态学报 2009年 29卷 1期 页码：53~58

关键词：小花棘豆；内生真菌；5.8S rDNA/ITS；分生孢子；

Keywords:Oxytropis glabra DC., endophytic fungi, 5.8S rDNA/ITS, conidia,

摘 要 ：小花棘豆(Oxytropis glabra DC.)是内蒙古草原上的重要毒草，实验结合微生物学和分子生物学手段进行了其内生真菌研究。结果表明：体外培养的小花棘豆内生真菌生长缓慢，呈圆形、隆起、边缘整齐、辐射状生长的白色菌落，后菌体分泌黑褐色的色素物质，分生孢子近圆柱形，有粗且比孢子壁厚的暗色横隔膜，隔膜数1~5个。10个菌株的5.8S rDNA/ITS序列与内生真菌Embellisia sp.L12株的序列高度相似。推测该内生真菌属于Embellisia。

Abstract:The Oxytropis glabra DC. is an important poisonous plant species in Inner Mongolia steppe. In this research the endophytic fungi from O.glabra DC. were studied both by microbiological and molecular biological techniques. The results showed as follows: The in vitro cultured endophytic fungi displayed round, raised, with uniform margin and radial growth colonies which grew slowly. Later a black brown pigment was secreted in each colony. The conidia were near cylindraceous with dark transverse septa thicker than conidial walls. The septa numbers varied from 1 to 5. The sequences of 5.8S rDNA/ITS from 10 strains of O.glabra DC. were highly similar to that of Embellisia sp.L12, which indicated the endophytes might belong to Embellisia.

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