---

作 者 ：李钧敏,金则新,钟章成

期 刊 ：生态学报 2004年 24卷 3期 页码：567~573

关键词：大血藤；RAPD；遗传变异；海拔高度；环境因子；

Keywords:Sargentodoxa cuneata, RAPD, genetic variation, altitude, environmental factors,

摘 要 ：利用 RAPD技术分析了分布于浙江省天台山 3个不同海拔高度的天然大血藤群体的遗传多样性、遗传分化以及与环境因子的相关性。 13种随机引物在 3 6株个体中共检测到 88个可重复的位点 ,其中多态位点 74个 ,总多态位点百分率为84.0 9% ,大血藤具有丰富的遗传多样性。 Shannon信息指数显示的遗传多样性以海拔 950 m的群体为最高 ,其次是海拔 73 0 m的群体 ,最低的是海拔 52 0 m的群体 ;群体内的遗传多样性占总遗传多样性的 43 .68% ,群体间的遗传多样性占 56.3 2 %。 Nei指数估计大血藤群体间的遗传分化系数为 0 .540 6,大血藤群体间的基因流很低。大血藤海拔 73 0 m群体与海拔 52 0 m群体的遗传相似度较高 ,海拔 950 m群体与其它两群体的遗传相似度较低。大血藤群体内的遗传多样性与土壤总氮呈极显著的正相关。

Abstract:The genetic diversity within populations and genetic differentiation among populations of Sargentodoxa cuneata at three different altitudes in Tiantai mountain of Zhejiang Province were analyzed by random amplified polymorphic DNA technique. To explore how S. cuneata fits environment, the relationships between the genetic diversity and the environmental factors were analyzed. 13 random primers were selected in the amplification and 88 repetitive loci with 74 polymorphic loci were produced. The total average...

---